云南南部不同种源地小桐子遗传多样性的ISSR分析  

Genetic Diversity of Jatropha curcas (Euphorbiaceae) Collected from Southern Yunnan, Detected by Inter-simple Sequence Repeat (ISSR)

作　　者：向振勇[1] 宋松泉[2] 王桂娟[2] 陈茂盛[2] 杨成源[2] 龙春林[1] 

机构地区：[1]中国科学院昆明植物研究所,云南昆明650204 [2]中国科学院西双版纳热带植物园,云南勐腊666303

出　　处：《云南植物研究》2007年第6期619-624,共6页Acta Botanica Yunnanica

基　　金：国家科技基础条件平台(2004DKA30430;2005DKA210006);云南省自然科学基金(2005C0053M)资助项目

摘　　要：应用ISSR分子标记方法对采自云南的8个居群的小桐子(Jatropha curcas)共158个个体进行遗传多样性分析。8个ISSR引物共扩增到了67个位点,其中61个是多态性位点。分析结果表明:(1)云南小桐子的遗传多样性水平很高。在物种水平上,平均每个位点的多态位点百分率PPB=91.04%,有效等位基因数Ne=1.5244,Nei′s基因多样性指数He=0.3070,Shannon多样性信息指数Ho=0.4618;在居群水平上,PPB=55.04%,Ne=1.3826,He=0.2171,Shannon多样性信息指数Ho=0.3178。(2)居群间的遗传分化低于居群内的遗传分化。基于Nei′s遗传多样性分析得出的居群间遗传多样性分化系数Gst=0.2944。AMOVA分析显示:云南小桐子的遗传变异主要存在于居群内,占总变异的63.50%,居群间的遗传变异占36.50%。(3)居群间的地理距离及遗传一致度并不存在相关性。鉴于以上指标,我们推测云南小桐子可能来自不同的地区。The genetic diversity of 158 individuals from eight semi-wild populations from Yunnan Province was estimated using ISSR method （8 primers）. The results revealed an extraordinarily high level of genetic diversity （at species level, percentage of polymorphic loci PPB = 91.04%, effective number of alleles Ne = 1.5244, Nei＇s （1973） gene diversity He =0.3070, and Shannon＇s information index Ho =0.4618; at population level, PPB = 55.04%, Ne = 1.3826, Nei＇s （1973） gene diversity He = 0.2171, and Shannon＇s information index Ho = 0.3178）. The level of genetic differentiation between populations is lower than that among populations. The low level of genetic differentiation among populations was detected, based on Nei＇s genetic diversity analysis （29.44%）, and AMOVA （36.50%）. ]here is no associations between geographical distance and genetic identity. We suggest that Jatropha curcas of Yunnan Province might not be introduced from the same place.

关 键 词：小桐子 遗传变异 ISSR 

分 类 号：Q943[生物学—植物学]