凋亡诱导因子的研究进展  

Progress in Apoptosis-inducing Factor

作　　者：陈默[1] 潘敏慧[1] 周恩昆[1] 杜娟[1] 刘佳[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与生物系统学研究所,重庆400716

出　　处：《细胞生物学杂志》2007年第6期791-794,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30471312);国家重点基础研究发展规划(973计划)(No.2005CB121000)资助项目~~

摘　　要：凋亡诱导因子(apoptosis-inducing factor,AIF)是一类存在于线粒体内外膜间隙的保守的黄素蛋白,具有双重功能。在细胞正常的生理状态下,作为线粒体氧化还原酶,能催化细胞色素c(Cytc)和NAD之间的电子传递,当细胞受到凋亡刺激后,就从膜间隙释放到细胞质中,并通过其核定位信号序列(nuclear localization sequence,NLS)进入细胞核内,引起染色体核周边凝集和DNA呈大片段断裂(约50kb),进而引发不依赖于caspase的细胞凋亡。AIF的释放受Bcl-2家族蛋白的调控,同时也受Hsp70的抑制,它还是多聚(ADP核糖)聚合酶1[poly(ADP-ribose)polymerase1,PARP1]介导的细胞凋亡途径的下游效应物。Apoptosis-inducing factor （AIF） is a conservative flavoprotein which has the difunctionality located in mitochondrial intermembrance space. It catalysises electron transmit between cytochrome c and NAD as mitochondrion oxidoreductase in cell normal physiological state. When death stimuli present, AIF is released from mitochondria to the cytoplasm and then to the nucleus through its nuclear localization sequence （NLS）, inducing peripheral chromatin condensation and large-scale fragmentation of DNA （～50 kb）, the last initiate caspase-independent pathway. The release of AIF is regulated by Bcl-2 proteins and inhibited by Hsp70. Moreover, AIF is a downstream effector in apoptosis pathway mediated by poly （ADP-ribose） polymerase 1 （PARP1）.

关 键 词：细胞凋亡 凋亡诱导因子 线粒体 

分 类 号：Q255[生物学—细胞生物学]