SRp38基因研究进展  

The Progress in Researches of SRp38 Gene

作　　者：彭正羽[1] 张薇[2] 陈献华[1] 徐平[1] 

机构地区：[1]复旦大学医学院医学神经生物学国家重点实验室,上海200032 [2]浙江大学城市学院药学系,杭州310015

出　　处：《细胞生物学杂志》2007年第6期821-825,共5页Chinese Journal of Cell Biology

基　　金：上海市卫生局科研基金资助项目(No.2006003)~~

摘　　要：SR蛋白在前体mRNA可变剪接调控中发挥重要作用。可变剪接调节因子SRp38作为一种新近发现的具有神经及生殖组织特异性的SR蛋白,有典型的SR蛋白结构特征并能够调控GluR-B、TRK-C以及NCAML1等基因的可变剪接,但与其他SR蛋白不一致的是,SRp38可以在一定条件下(有丝分裂M期,热休克)抑制前体mRNA剪接,从而防止错误剪接的出现。SRp38的RRM结构域可以识别特殊的RNA序列并跟U1snRNP结合,而其RS结构域则参与调控前体mRNA剪接。SRp38的磷酸化状态可以影响其调控功能的发挥,在有丝分裂M期及热休克时,该蛋白质均呈去磷酸化状态。SRp38在爪蟾胚胎神经发育过程中发挥作用并且可以同TLS(translocation liposarcoma)蛋白相互作用,提示其可能通过调节前体mRNA可变剪接在神经系统的发育分化以及在肿瘤的发生中扮演角色。SR proteins play important role in regulating pre-mRNA alternative spicing. As a newly discovered neural and reproductive specific SR protein, SRp38 has typical structure of SR protein and can regulate alternative splicing of several genes such as GLUR-B, TRK-C and NCAML1 etc. But unlike the other SR proteins, during mitosis or stress response, SRp38 can act as splicing inhibitor in order to prevent wrong splicing. The RRM motif of SRp38 can recognize specific mRNAs sequence and the RS domain is responsible for the activity of the modified exon inclusion. The physiological function of SRp38 is regulated by its phosphorylation status, it is dephosphorylated in M phase cells or after heat shock. SRp38 plays important role in neuronal differentiation during early embryogenesis and can also interaction with TLS hint its function as a regulator in neurogenesis as well as carcinogenesis.

关 键 词：SRp38 前体mRNA可变剪接 磷酸化 调控 

分 类 号：Q343[生物学—遗传学]