孕鼠血清高胆酸水平对胎鼠大脑组织形态的影响  

Effects of high cholic acid on fetal brains of pregnant rats

作　　者：谭丽[1] 丁依玲[1] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011

出　　处：《中华妇产科杂志》2007年第12期840-845,共6页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨孕鼠血清高胆酸水平对胎鼠大脑组织形态的影响。方法将30只清洁级成年 SD 孕鼠随机分为 A、B、C 3组,每组10只。在妊娠第13～20天,A 组孕鼠每天1次腹腔注射纯胆酸5.5 mg·kg^(-1)·d^(-1)(高胆酸);B 组孕鼠每天1次腹腔注射纯胆酸1.4 mg·kg^(-1)·d^(-1)(低胆酸);C 组同步注射等量生理盐水。妊娠第21天剖腹取胎,测定胎鼠体重及记录死亡率。测定孕鼠和胎鼠血清总胆酸水平,采用酶联免疫吸附试验测定血神经元特异烯醇化酶(NSE)水平。同时,胎鼠断头取脑,采用光镜和电镜观察胎鼠大脑组织的病理改变。结果 (1)3组孕鼠及胎鼠血清胆酸水平分别为:A 组(22.3±8.1)μmol/L,(28.8±8.1)μmo]/L;B 组(9.8±3.6)μmol/L,(9.3±3.5)μmol/L;C组(3.6±1.8)μmol/L,(4.0±1.2)μmol/L。3组间相互比较,差异有统计学意义(P<0.01)。孕鼠血清总胆酸水平与胎鼠血清总胆酸水平呈正相关关系(r=0.875,P<0.01)。(2)胎鼠死亡率在 A、B、C组中分别是30.1%,16.9%和7.1%,3组间相互比较,差异有统计学意义(P<0.05)。(3)A、B、C 组胎鼠血清 NSE 水平分别为(31.9±13.1),(13.9±5.9)和(9.3±3.9)ng/L,A 组明显高于 B 组及 C 组(P<0.01);B 组与 C 组比较,差异无统计学意义(P>0.05)。胎鼠血清 NSE 水平与总胆酸水平呈正相关(r=0.758,P<0.01)。(4)胎鼠大脑皮层组织光镜下病理改变:A、B 组胎鼠均表现为不同程度的大脑皮层组织层次紊乱,神经元变性坏死,密度降低,胞核固缩深染。胎鼠神经元变性面积积分在A、B、C 组中分别是(1.4±0.6),(1.5±0.7)和(0.7±0.3),A 组和 B 组明显高于 C 组(P<0.01);A组与 B 组比较,差异无统计学意义(P>0.05)。胎鼠神经元变性面积积分与胎鼠血清 NSE 水平的r为0.282(P>0.05)。胎鼠神经元坏死面积积分在 A、B、C 组中分别是(1.8±0.7),(0.9±0.4)和(0.6±0.3),A 组明显高于 B 组和 C 组(P<0.05);B 组与 C 组比较,差异无统计学意义(P>0.05)。胎鼠神经元坏死面积积分与胎Objective To investigate the effects of morphous on fetal brains in pregnant rat of high cholic acid. Methods Randomly deviding 30 SD pregnant rats to three groups A, B and C, every group is 10. From 13^th to 20^th days of pregancy, injecting 5.5 mg·kg^-1·d^-1 cholic acid to pregnant rats of group A, 1.4 mg·kg^-1·d^-1 cholic acid to group B and the partes aequales normal saline to group C by intraperitoneal injection one time every day. In the 21^st day of pregancy, to cut the belly open and take the fetus out and record the total fetus, live fetus and the weight. Determine the serum concentration of total bile acid （TBA） in pregnant rats and fetal rats. Enzyme linked immunosorbent assay（ELISA） was used to detect the serum level of neuron-specific enolase（NSE） in fetal rats. Fix and embed the brain after decapitation, then to observe the pathological change of the fetal cerebrum under light microscope and electron microscope. Results （ 1 ） The serum concentration of TBA of pregnant rats and fetal rats in group A is （22. 3 ± 8. 1 ） μmol/L and （28. 8 ± 8. 1 ） μmol/L, in group B is （9. 8 ± 3.6） μmol/L and （9. 3 ± 3.5 ） μmol/L, in group C is （3.6 ± 1.8） μmol/L and （4. 0 ± 1.2） μmol/L. There is significant defference in every two groups, P 〈 0.01. The serum concentration of TBA between pregnant rats and fetal rats were positively correlated with each other, （ r = 0. 875, P 〈 0. 01 ）. （2） The mortinatality of fetus in group A, B and C are 30. 1%, 16. 9% and 7. 1% , there is significant defference in every two groups, P 〈0. 05. （3） The serum lever of NSE of fetus in group A was significantly higher than that of group B and C, [ （31.9 ± 13. 1 ） ng/L vs （ 13.9 ± 5.9 ） ng/L and （9. 3 ± 3.9） ng/L, both are P 〈 0. 05 ]. But there is no significant difference between group B and C, P 〉 0. 05. The serum level of TBA and NSE in fetus were positively correlated with each other, （ r = 0. 758, P 〈0. 01 ）. （4） By the light microsc

关 键 词：妊娠 动物 胆酸 磷酸丙酮酸水合酶 脑 显微镜检查 大鼠 Sprague-Dawley 

分 类 号：R714.2[医药卫生—妇产科学]