Phenotypic and Functional Analysis of LCMV gp33-41-Specific CD8 T Cells Elicited by Multiple Peptide Immunization in Mice Revealed the Up-regulation of PD-1 Expression on Antigen-Specific CD8 T Cells  

作　　者：Yi Liu Lihui Xu Yiqun Jiang Jianfang Sun Xianhui He 

机构地区：[1]Institute of Dermatology, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Nanjing 210042, China [2]Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China [3]Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

出　　处：《Cellular & Molecular Immunology》2007年第6期431-437,共7页中国免疫学杂志（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.30672474,No.30572199,No.30230350,and No.30371651);grants from Institute of Dermatology,Chinese Academy of Medical Sciences&Peking Union Medical College.

摘　　要：The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination. Here we showed that multiple immunizations with LCMV gp33-41 peptide （KAV） in Freund＇s adjuvant could induce KAV-specific CD8 T cells with low expression of CD127 and CD62L molecules. The inhibitory receptor PD-1 was also expressed on a substantial part of KAV-specific CD8 T cells, and its expression level on KAV-specific CD8 T cells in spleen and lymph nodes was much higher when compared to those in peripheral blood. Furthermore, KAV-specific CD8 T cells could specifically kill KAV-pulsed target cells in vivo but the efficiency was low. These data suggest that prime-boost vaccination schedule with peptide in Freund＇s adjuvant can elicit antigen-specific CD8 T cells of effector-like phenotype with partial functional exhaustion, which may only provide short-term protection against the pathogen. Cellular ＆ Molecular Immunology.The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination. Here we showed that multiple immunizations with LCMV gp33-41 peptide （KAV） in Freund＇s adjuvant could induce KAV-specific CD8 T cells with low expression of CD127 and CD62L molecules. The inhibitory receptor PD-1 was also expressed on a substantial part of KAV-specific CD8 T cells, and its expression level on KAV-specific CD8 T cells in spleen and lymph nodes was much higher when compared to those in peripheral blood. Furthermore, KAV-specific CD8 T cells could specifically kill KAV-pulsed target cells in vivo but the efficiency was low. These data suggest that prime-boost vaccination schedule with peptide in Freund＇s adjuvant can elicit antigen-specific CD8 T cells of effector-like phenotype with partial functional exhaustion, which may only provide short-term protection against the pathogen. Cellular ＆ Molecular Immunology.

关 键 词：LCMV CD8 T cell peptide vaccine phenotype PD-1 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]