流感病毒A/FM/47(H1N1)血凝素基因克隆、序列分析  

Cloning and Sequencing of HA Gene of Avian Influenza Virus Strain A/Font Monmouth/47(H1N1)

作　　者：王艳芳[1] 闵晓莉[2] 杨子峰[3] 何金洋[4] 孙立[5] 王新华[4] 

机构地区：[1]华南师范大学激光运动医学实验室,广州510006 [2]广东省中医院,广州510120 [3]广州医学院第一附属医院广州呼吸疾病研究所,广州510120 [4]广州中医药大学,广州510405 [5]暨南大学,广州510630

出　　处：《热带医学杂志》2007年第12期1153-1154,1193,共3页Journal of Tropical Medicine

摘　　要：目的对流感病毒A/Font Monmouth/47(H1N1)血凝素基因进行变异分析。方法用SPF鸡胚增殖毒株,提取病毒基因组总RNA,应用RT-PCR技术扩增该病毒株的HA基因,并克隆到pMD18-T载体上,测定HA基因核苷酸序列。结果该毒株的HA基因全长为1677bp,含有完整的阅读框架,编码545个氨基酸;BLAST序列比对结果显示,该毒株HA基因与其他H1基因核苷酸的同源性为98%。结论该毒株HA核苷酸序列发生了变异,出现多处碱基替换,可能是病毒毒力增强的原因。Objective To clone the hemagglutinin （HA） gene from influenza A （H1N1）. Method HA gene was amplified using specific primers and RT-PCR according to the sequence published on Genbank. The amplified fragment was cloned into pMD18-T vector for sequence analysis. Result The cloned HA gene shared 98% nucleotide sequence homology with the other H1N1 subtypes. Conclusion The results confirmed the high frequency of recombination of the influnenza A virus genome and the high level of genetic variation variation of Influenza A.

关 键 词：流感病毒 血凝素 基因序列 

分 类 号：R373.13[医药卫生—病原生物学]