三七三醇皂苷对大鼠局灶性脑缺血损伤的保护作用和脑血流量的影响  

Effects of PTS on Focal Cerebral Ischemia and Cerebral Blood Flow in Rat

作　　者：陈健文[1] 蓝秀健[2] 陈少锐[1] 马仁强[1] 徐索文[1] 赖文珊[1] 周小石[1] 刘培庆[1] 

机构地区：[1]中山大学药学院,广州510080 [2]中山大学中山医学院,广州510080

出　　处：《热带医学杂志》2007年第12期1159-1161,1201,F0004,共5页Journal of Tropical Medicine

基　　金：广东省重点攻关基金(No2003C30113);中山大学实验室开放基金(NoKF200641)

摘　　要：目的探讨三七三醇皂苷(PTS)对局灶性脑缺血损伤的保护作用。方法将大鼠随机分成6组:假手术组,模型组,PTS25、50、100mg/kg组和阳性对照药血栓通注射液(XST)组;采用栓线法使大脑中动脉闭塞(MCAO)建立大鼠局灶性脑缺血模型,于造模24h后进行行为学评分,测定脑组织含水量以及脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量,计算脑梗塞面积百分比以及进行组织病理学检查。结果三七三醇皂苷50、100mg/kg能明显缓解脑缺血大鼠的行为学症状,减轻其脑水肿程度,降低血清中MDA含量和升高SOD含量,并能减少其脑梗塞面积,减轻脑组织病理学损伤。结论PTS对大鼠局灶性脑缺血有较明显的保护作用,增加脑血流量可能为治疗作用机理之一。Objective To study the protective effects of PTS on focal cerebral ischemia in rats. Method Rats were randomly divided into 6 groups： the Sham, the Control, the PTS （25 mg, 50 mg and 100 mg/kg）, and the XST. The rat＇s mode of focal cerebral ischemia was made by middle cerebral artery occlusion （MCAO） with nylon suture; the effects of the drug were studied about neurological deficit, the water content of brain tissue, the activities of SOD , and MDA in brain tissue, the cerebral infracted zone, as well as undermicroscopic examination. Result PTS （50 mg and 100 mg/kg） could significantly reduce the scores for neurological deficits and brain edema, improve the activities of SOD, lower the content of MDA, reduce the cerebral infracted zone and alleviate the injury of cerebral ischemia. Conclusion PTS has obviously protective effects on the focal cerebral ischemia in rats, the increase of rCBF may be one of the protection mechanism.

关 键 词：脑缺血 大脑中动脉闭塞 三七三醇皂苷 局部脑血流量 

分 类 号：R969.4[医药卫生—药理学] R743.9[医药卫生—药学]