苦豆子总碱对大鼠溃疡性结肠炎细胞因子IL-10表达的影响  

Effects of total base of sophora alopecuroides on expression of IL-10 cytokine in rats with experimental colitis

作　　者：周毅[1] 邓虹珠[1] 朱学敏[1] 陈静娴[1] 邓静君[1] 曾镇[1] 

机构地区：[1]广州医学院药理教研室,510182

出　　处：《国际消化病杂志》2007年第6期465-467,469,共4页International Journal of Digestive Diseases

基　　金：广州市科技计划项目资助(编号:2006Z3-E5051-2)

摘　　要：目的观察苦豆子总碱对大鼠溃疡性结肠炎(UC)的外周血和结肠组织中IL-10表达的影响。方法将SD大鼠随机分成6组(正常组、模型组、柳氮磺胺吡啶(SASP)、苦豆子总碱高剂量组、中剂量组、低剂量组),采用三硝基苯磺酸(TNBS)灌肠法,制备结肠炎大鼠模型。第2天开始灌胃,在第3周杀鼠后用酶联免疫吸附法(ELISA)检测IL-10在结肠黏膜组织和外周血清中的表达水平;同时分析结肠黏膜组织IL-10与DAI(疾病活动指数评分)/组织学损伤的相关性。结果(1)结肠部位和外周血清中IL-10的表达较对照组均显著降低,其中以结肠部位降低的幅度最大(P<0.01)。(2)苦豆子总碱高、中、低3个剂量组和SASP组都能显著性上调结肠部位和外周血清的IL-10的表达(P<0.05),且结肠部位的变化与外周血的相比差异明显。(3)结肠部位的DAI与结肠IL-10之间存在的负相关性(Pearsonr=-0.828,P<0.01),结肠部位的组织学损伤与结肠IL-10之间存在负相关性(Pearsonr=-0.819,P<0.01);所有检测为双侧,样本容量n=60。结论苦豆子总碱通过上调IL-10的表达减轻或改善实验性结肠炎大鼠的组织学损伤和症状。Objective To investigate the effects of total base of sophora alopecuroides （TBSA） on expression of IL-10 cytokine in rats with experimental colitis. Method SD rats were randomized into 6 groups：normal control group, the model group, SASP group and 60 mg/kg, 30mg/kg, 15mg/kg TBSA group with 10 rats in each group, and UC was induced by administrating 2,4,6 trinitrobenzesulphonic acid （TNBS） in rats. Rats were given drug by stomach after the first day for 3 weeks, and sacrificed at 23 day to determine the expression of IL-10 cytokine in colonic mucosa and peripheral blood in order to investigate the effects of TBSA in UC rats by ELISA. Results The expression of IL-10 cytokine on the colon of UC rats is lower than that of the control （P〈0.01）, especially, the expression on colon tissue was the lowest in the them. the expression of IL-10 cytokine on the cured rats with TBSA was almost as well as that of the control （P〈0.05）. There is negative correlation between the expression of IL-10 and DAI in the colon of UC rats（Pearson r= -0. 828,P〈0.01）,and is negative correlation between the expression of IL-10 and histological grading in the colon of UC rats（Pearson r = -0. 819,P〈0.01 ）. Conclusion TBSA can raise the expression of IL-10 in order to improve the symptom with UC.

关 键 词：苦豆子总碱 IL-10 溃疡性结肠炎 酶联免疫吸附法 

分 类 号：R285.5[医药卫生—中药学]