内源性硫化氢抑制高肺血流大鼠肺动脉弹力蛋白的表达  

Endogenous hydrogen sulfide inhibits expression of elastin of pulmonary artery with high pulmonary blood flow in rats

作　　者：王聪[1] 刘文莉[1] 王海英[1] 涂家红[1] 李晓惠[2] 赵斌[1] 杜军保[3] 

机构地区：[1]北京大学第四临床医院北京积水潭医院急诊科,北京100035 [2]首都儿科研究所儿童医院,北京100020 [3]北京大学第一医院儿科,北京100034

出　　处：《基础医学与临床》2007年第12期1329-1333,共5页Basic and Clinical Medicine

基　　金：北京市自然科学基金(7052043)

摘　　要：目的探讨内源性硫化氢(H2S)对高肺血流所致肺血管重构大鼠肺动脉弹力蛋白的调节作用。方法将雄性SD大鼠随机分为4组:分流组、分流+炔丙基甘氨酸(PPG)组、假手术组和假手术+PPG组,每组8只。分流组及分流+PPG组大鼠经腹主动脉-下腔静脉穿刺建立高肺血流动物模型。4周后,测定大鼠肺动脉平均压(mPAP),显微镜下观察肺血管结构变化;用免疫组化方法测定α-平滑肌肌动蛋白(α-SM-actin)、弹力蛋白、转化生长因子-β(TGF-β)和结缔组织生长因子(CTGF)在肺动脉平滑肌细胞的表达。结果分流+PPG组mPAP较分流组和假手术组明显升高(P<0.05);在分流组,小型肺动脉中肌型动脉和部分肌型动脉所占百分比明显高于假手术组(均P<0.05),非肌型动脉所占百分比明显低于假手术组(P<0.01);在分流+PPG组,小型肺动脉中肌型动脉所占百分比明显高于分流组(P<0.01),非肌型动脉所占百分比明显低于分流组(P<0.01)。分流组肺中,小型肺动脉平滑肌细胞α-SM-actin、弹力蛋白、TGF-β和CTGF含量明显高于假手术组(均P<0.01),分流+PPG组上述指标明显高于分流组(均P<0.01)。结论内源性硫化氢可能通过抑制高肺血流大鼠肺动脉弹力蛋白的表达,调节其肺血管结构重构。Objective To investigate the regulatory effect of endogenous hydrogen sulfide （ H2S） on the expression of elastin in rats with pulmonary vascular structural remodeling induced by high pulmonary blood flow. Methods Thirty-two male SD rats were randomly divided into four groups （ 8 rats in each） ： shunt group, shunt ＋ PPG （ propargylglycine） group, sham group and sham ＋ PPG group. Each rat in shunt and shunt ＋ PPG group was subjected to an aorta-caval shunting operation to cstablish a rat model of high pulmonary blood flow. The rats in the shunt ＋ PPG and sham ＋ PPG groups were intraperitoneally injected with PPG. After 4 weeks, the mean pulmonary arterial pressure （mPAP） was measured. The percentage of muscularized arteries of small pulmonary arteries of four group rats was measured by microscopy. The expression of α-smooth -muscle actin, elastin, TGF-β, and CTGF in pulmonary artery smooth muscle ceils was observed by immunohistochemistry method. Results The mPAP of the shunt ＋ PPG group was significantly higher than that of the shunt group and sham group （ P 〈 0. 05 ）. The percentage of muscularized arteries and partly muscularized arteries of small pulmonary arteries was increased in rats of shunt group as compared to sham group （ P 〈 0. 05 ）. While, the percentage of non-muscularized arteries of small pulmonary arteries decreased in rats of shunt group as compared with that of sham group （P 〈0. 01 ）. However, when PPG was used, the percentage of muscularized arteries of small pulmonary arteries increased in rats of shunt ＋ PPG group as compared to that of shunt group （P 〈0. 01 ）. The percentage of non-muscularized arteries of small pulmo- nary arteries decreased in rats of shunt ＋ PPG group as compared with that of shunt group （P 〈0. 01 ）. The expression of or-smooth-muscle actin, elastin, TGF-β and CTGF of median and small pulmonary artery smooth muscle ceils in shunt group significantly increased as compared to that in sham group （P 〈 0. 0

关 键 词：硫化氢 高肺血流 血管结构重构 弹力蛋白 转化生长因子-Β 

分 类 号：R543[医药卫生—心血管疾病]