短期运动对大鼠缺血/再灌注心肌的保护作用  

Protective effect of short-term exercise on ischemic/reperfused myocardium in rats

作　　者：侯云英[1] 范秀珍[1] 马志勇[2] 

机构地区：[1]山东大学护理学院,山东济南250012 [2]山东大学教育部和卫生部心血管重构与功能研究重点实验室,山东济南250012

出　　处：《基础医学与临床》2007年第12期1357-1359,共3页Basic and Clinical Medicine

基　　金：山东大学青年基金(21330051310061)

摘　　要：目的研究短期中等强度运动训练对大鼠缺血/再灌注(I/R)心肌的保护作用及与蛋白激酶C(PKC)激活的相关性。方法40只Wistar大鼠随机分为4组(每组10只):对照组(CON组)、运动组(EXE组)、运动+PKC抑制剂组(E+C组)和PKC抑制剂组(CHE组)。应用Langendorff离体心脏I/R模型,观察各组大鼠心脏再灌注期间心律失常的发生情况、心功能指标恢复率及心肌梗死范围。结果EXE组大鼠LVDP(再灌注30、60 min)和RPP(再灌注20、30、60 min)恢复率高于CON和E+C组(P<0.05或P<0.01),心肌梗死范围显著低于CON和E+C组(P<0.01)。CON、CHE组上述各指标无差异。结论短期中等强度运动训练对I/R心肌有保护作用,保护作用与PKC的激活有关。Objective To investigate the protective effect of short-term moderate exercise on ischemic/reperfused myocardium and its correlation to the activation of protein kinase C （PKC）. Methods Fourty Wistar rats were randomly divided into 4 groups （n = 10 respectively） ： control group（ CON group）, exercise group（ EXE group）, exercise ＋ PKC inhibitor group（ E ＋ C group） and PKC inhibitor group（ CHE group）. The occurrence of arrhythmia, the recovery of cardiac function, and infarct size were observed by using the Langendorff-ischemia/reperfusion model in isolated rat heart in vitro. Results Recovery rate of LVDP （on the 30th and 60th minute of reperfusion） and RPP（on the 20th, 30th and 60 minute of reperfusion） of EXE group were higher than those of CON and E ＋ C groups （ P 〈 0. 05 ）, infarct size of EXE group was dramatically lower than that of CON and E ＋ C groups （P 〈 0. 01 ）, but the difference was not sienificant. Conclusion Short-term and moderate exercise protects myocardium from ischemia/reperfusion injury, the mechanism involves the activation of PKC.

关 键 词：运动 缺血/再灌注 心肌保护 蛋白激酶C 

分 类 号：R541[医药卫生—心血管疾病]