茯苓菌核多糖的分离和结构分析  

Isolation and Structural Analysis of Polysaccharides from the Sclerotium of Poria Cocos Wolf

作　　者：张俐娜[1] 丁琼 张平义 冯汉桥[2] 

机构地区：[1]武汉大学化学系,武汉430072 [2]中国科学院武汉物理研究所,武汉430072

出　　处：《高等学校化学学报》1997年第6期990-993,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：分别用0．9％NaCl水溶液、热水、0．5mol／LNaOH水溶液和88％甲酸从新鲜茯苓菌核体中提取出5种多糖：PC1、PC2、PC2－A、PC3和PC4．用IR、HPLC、GC、1HNMR和13CNMR等方法分析了它们的组成和结构．结果表明，PC1、PC2和PC2－A由D-葡萄糖、D-木糖、D-甘露糖和D-半乳糖组成，PC1和PC2－A含葡萄糖醛酸，其葡萄糖含量随提取过程的进行逐渐增加．PC3主要由线型β-（1→3）－D-葡聚糖组成，它占茯苓多糖的绝大部分．PC4由β-（1→3）－D-葡聚糖组成，含少量支链和葡萄糖醛酸．Five polysaccharides (PC1, PC2, PC2-A, PC3 and PC4) were isolated from a fresh sclerotium of Poria cocos by using 0. 9% NaCl aqueous solution, hot water, 0. 5 mol/L NaOH aqueous solution and 88% formic acid, respectively. The components and structures of the polysaccharides were analyzed by IR, GC, HPLC, 13C NMR and 1H NMR. The polysaccharides of PC1, PC2 and PC2-A consist of D-glucose, D-xylose, D-mannose and D-galactose, and the D-glucose content increases with the progress of isolation process. The PC1 and PC2-A contain glucuronic acid. The polysaccharide PC3 contains mainly linearβ-(1-3)-D-glucan, and is the major fraction of Poria cocos polysaccharides. The PC4 is com-posed of β-(1-3)-D-glucan with a few of braching and glucuronic acid.

关 键 词：茯苓菌核 多糖 结构 核磁共振 组成 分离 

分 类 号：R284.2[医药卫生—中药学]