长蒴母草黄脉病毒——一个双生病毒的新种  

Molecular characterization of a distinct Begomovirus infecting Lindernia anagallis in China

作　　者：车海彦[1] 刘先宝[1] 杨旭光[1] 符瑞益[1] 罗大全[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,儋州571737

出　　处：《植物病理学报》2007年第6期578-583,共6页Acta Phytopathologica Sinica

基　　金：中国热带农业科学院科技基金(Rky0303)

摘　　要：从采集于海南儋州地区表现黄脉症状的长蒴母草(Lindernia anagallis)上分离到病毒分离物L2,DNA-A全序列分析结果表明,全长2739个核苷酸(nt)(GenBank登录号:AY795900),共编码6个ORF,其中病毒链编码AV1(CP)、AV2,互补链编码AC1、AC2、AC3、AC4。利用BLAST程序对DNA-A进行分析表明,与L2 DNA-A有同源关系的病毒均为双生病毒科(Geminiviridae)菜豆金色黄花叶病毒属(Begomovirus)成员。进一步比较发现,L2 DNA-A与我国广东报道的广东番茄曲叶病毒(Tomato leaf curl Guangdong virus,ToLCGuV)(AY602165)全基因组核苷酸序列的同源性最近,仅为77.0%,说明L2为Begomovirus中的一个新种,命名为长蒴母草黄脉病毒(Lindernia anagallis yellow vein virus,LAYVV)。与L2的IR区及各基因编码的氨基酸序列有最高同源性的病毒均来源于亚洲。利用DNA-B特异引物和DNA-β的特异引物,均未检测到DNA-B和卫星DNA-β的存在。The isolate L2 was obtained from Lindernia anagallis showing yellow mosaic symptom in Hainan province, China. The complete nucleotide sequence of DNA-A-like molecule of L2 contained 2 739 nucleotides and encoding six ORFs with two ORF, AV1 （CP） and AV2 in virion-sense DNA, AC1, AC2, AC3 and AC4 in complementary-sense DNA. L2 DNA-A had the highest sequence identity （77.0%） with that of Tomato leaf curl Guangdong virus （ToLCGuV, accession number AY602165）. Based on the molecular data, L2 was considered as a new Begomovirus species, for which the name Lindernia anagaUis yellow vein virus （LAYVV） was proposed. The individual ORF encoded by L2 DNA-A and IR region share the highest amino acid sequence identifies with Begomovirus from Asia. Attempts to amplify the putative DNA-B or satellite DNA-β with conserved primer pairs by PCR had been unsuccessful.

关 键 词：长蒴母草黄脉病毒 长蒴母草 菜豆金色黄花叶病毒属 基因组 

分 类 号：S432.41[农业科学—植物病理学]