菊欧文氏菌分子检测技术的研究  

Molecular detection of Erwinia chrysanthemi

作　　者：刘鹏[1] 黄国明[1] 刘勇[1] 崔铁军[1] 

机构地区：[1]天津出入境检验检疫局,天津300456

出　　处：《植物病理学报》2007年第6期584-587,共4页Acta Phytopathologica Sinica

摘　　要：蝴蝶兰细菌性软腐病对蝴蝶兰的生长危害严重,Erwinia chrysanthemi(菊欧文氏菌)、Erwinia carotovora subsp. carotovora(胡萝卜软腐欧文氏菌胡萝卜软腐亚种)是引起蝴蝶兰软腐病的主要病原细菌,其中E.chrysanthemi被列入我国三类检疫性有害生物。本文对菊欧文氏菌分子检测技术进行了研究,设计出针对该病原细菌的特异性引物,应用实时荧光PCR方法检测样品中存在的菊欧文氏菌,检测灵敏度达到102cfu/mL。Bacterial soft rot causes great loss to the growth of butterfly orchid. Erwinia chrysanthemi and Erwinia carotovora subsp, carotovora are the main bacterial pathogen led to rot disease. In the study, the molecular technology had been primarily established to detect E. chrysanthemi, the potential serious quarantine pathogen. The specific primers was designed to the detection of E. chrysanthemi and real-time fluorescent PCR method could achieve high sensitivity.

关 键 词：菊欧文氏菌 特异性引物 检测 

分 类 号：S432.42[农业科学—植物病理学]