烟草青枯病内生拮抗菌的筛选、鉴定及其防效测定  

Screening,identification and control efficacy of tobacco antagonistic endophytic bacteria against Ralstonia solanacearum

作　　者：彭细桥[1] 周国生 邓正平 匡传富 罗宽[2] 刘红艳[3] 

机构地区：[1]湖南省烟草公司郴州市公司烟科所,郴州423000 [2]湖南农业大学,长沙410128 [3]湖南省茶叶研究所,长沙410125

出　　处：《植物病理学报》2007年第6期670-674,共5页Acta Phytopathologica Sinica

基　　金：湖南省烟草专卖局基金资助项目(HN-02-006)

摘　　要：One hundred and sixty endophytic bacteria strains were isolated from healthy tobacco plants in severely diseased plots in Guiyang county,Hunan province.Three endophytic bacteria strains: H-1,H-9 and H-11 showed relatively strong antagonistic against Ralstonia solanacearum.Strain H-1 was identified as Bacillus subtilis,while strains H-9 and H-11 were identified as Bacillus brevis by their morphological,physiological and biochemical characteristics.Results from several experiment trials showed that the endophytic bacteria could reduce the disease incidence of tobacco.The control efficiency reached from 84.3% to 98.7%,it indicated that biocontrol with endophytic bacteria was an alternative and potential method to control tobacco bacterial disease.One hundred and sixty endophytic bacteria strains were isolated from healthy tobacco plants in severely diseased plots in Guiyang county, Hunan province. Three endophytic bacteria strains. H-1, H-9 and H- 11 showed relatively strong antagonistic against Ralstonia solanacearum. Strain H-1 was identified as Bacillus subtilis, while strains H-9 and H-11 were identified as Bacillus brevis by their morphological, physiological and biochemical characteristics. Results from several experiment trials showed that the endophytic bacteria could reduce the disease incidence of tobacco. The control efficiency reached from 84.3% to 98.7%, it indicated that biocontrol with endophytic bacteria was an alternative and potential method to control tobacco bacterial disease.

关 键 词：烟草青枯病 防效测定 拮抗菌 筛选 植物体内 生物防治 鉴定 内生细菌 

分 类 号：S572.034[农业科学—烟草工业]