鸡Galnact-2基因c.363C>G位点的多态性及其与体重、脂肪沉积性状的相关性  

Genetic Diversities of the Galnact-2 c.363C>G Mutation and Its Association with Body Weight and Fat Deposition in Chicken

作　　者：刘三凤[1] 聂庆华[2] 张细权[2] 张志燕[1] 任军[1] 黄路生[1] 

机构地区：[1]江西农业大学动物生物技术国家重点实验室培育基地,南昌330045 [2]华南农业大学动物科学学院,广州510642

出　　处：《农业生物技术学报》2007年第6期931-935,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30425045和30400312)资助。

摘　　要：β-1.4-N-乙酰半乳糖胺转移酶(β-1.4-N-acetylgalactosaminyl-transferase,Galnact-2)是催化O-聚糖合成第一步的糖基转移酶。根据已公布的鸡Galnact-2基因的DNA序列,在突变位点c.363C>G的侧翼(区)设计引物,用PCR-XhoⅠ-RFLP方法在6个鸡(Gallus gallus)品种:隐性白洛克鸡、杏花鸡、泰和丝毛乌骨鸡、白耳黄鸡、康乐鸡和南城黑鸡276个体中检测其多态性,并运用华南农业大学杏花鸡与隐性白洛克鸡资源群全同胞参考家系研究这个突变对鸡体重和脂肪沉积性状的影响。结果表明:(1)该突变位点在各品种中具有丰富的多态性;(2)该位点与鸡的体重显著相关。GG型个体的体重比CC型大,两者在出生重及21、28、35、49、56、63、77和84日龄体重差异极显著(P<0.01);GC型与CC型之间的出生重及21、56和63日龄体重差异极显著(P<0.01),35、49、77和84日龄体重差异显著(P<0.05);GG型与GC型个体体重差异不显著。(3)各种基因型个体的皮下脂肪厚、腹脂重、脂肪带宽、胸肌粗脂肪含量、腿肌粗脂肪含量等5种脂肪沉积性状之间均未见显著性差异。β-1.4-N-acetylgalactosaminyl-transferase （Galnact-2） is responsible for the first committed step in the synthesis of mucin-type O-glycans on protein substrates. In this study, primers flanking the ga/nact2 c.363 C〉G mutation were designed according to the known chicken galnact-2 DNA sequence. This polymorphism was genotyped in 276 animals from six chicken breeds（ Gallus gallus） including White Recessive Rock, Xinghua, Taihe Silk, Baier Huang, Kangle and Nancheng Black breeds, and in a F2 full sib resource population by crossing White Recessive Rock with Xinghua using PCR-XhoI-RFLP. The results showed that the genetic polymorphism of the Galnact-2 c.363C〉G mutation was abundant; This polymorphism was significantly associated with body weight at all ages measured. Individuals with the GG genotype had heavier body weight （BW） at the ages of 1, 21, 28, 35, 49, 56, 63, 77 and 84 days than those with the CC genotype （P 〈 0.01）. GC individuals had heavier BW at the ages of 1, 21, 56 and 63 days （P 〈 0.01）, and 35, 49, 77 and 84 days （P 〈 0.05） than that of CC individuals. There was no significant difference between GC and GG animal BW at all ages measured; And this polymorphism was not significantly associated with fatness traits including fat pad under the skin, abdominal fat weight, width of fat band, crude fat content in breast muscle and crude fat content in leg muscle.

关 键 词：鸡 β-1.4-N-乙酰半乳糖胺转移酶 遗传多样性 体重 脂肪沉积性状 

分 类 号：S188[农业科学—农业基础科学]