优化的VHb基因和融合杀虫基因在烟草中的表达  

Expression of Modified VHb Gene and Fusedly- insectidal Genes in Tobacco

作　　者：王友如[1] 崔洪志[2] 郭三堆[2] 

机构地区：[1]湖北师范学院,黄石435002 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《农业生物技术学报》2007年第6期982-986,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2001AA222101)资助。

摘　　要：将人工合成的、密码子优化后的透明颤菌血红蛋白(VHb)基因(vgbM)与含有融合杀虫基因(GFMcryIA和CPTI)表达载体pGBIF4ABC构建成双价基因植物高效表达载体pGBIF4ABCVHB,通过根癌农杆菌(Agrobacterium tumefaciens)介导转化烟草(Nicotiana tabacum),经PCR及Southernblot检测,证实了双价基因在32株转基因烟草基因组中整合。Westernblot检测证实了vgbM基因的表达,杀虫实验表明,融合杀虫基因表达的毒蛋白具有杀虫活性,转基因烟草平均每株干重高于非转基因烟草植株8%。表明VHb基因和融合杀虫基因烟草在烟草中的表达可使烟草可增产,又具抗虫性。The highly-efficient plant binary expression vector pGBIF4ABCVHB was constructed by applying the artificially-modified-synthesized vgbM gene and fusedly-insectidal genes （GFMcryIA and CPTI）. Transgenic tobacco （Nicotiana tabacum） plants were obtained by Agrobacterium mediated transformation, 32 of them were identified that the bivalent genes were inserted into the tobacco genome by PCR amplification detection and Southern blotting analysis. Western blotting analysis proved that vgbM gene was expressed in the transgenic plants. Toxicity assay indicated that fused insectidal genes were expressed activity of pestieadal toxin protein.The net weight of transgenie tobacco plants exceeded 8% of that of nontransgenie ones. Compared to non- transgenic tobacco plants, the transgenic ones were high-yield varieties of insect-resistance.

关 键 词：VHB基因 融合杀虫基因 转基因植株 抗虫性 产量 

分 类 号：S188[农业科学—农业基础科学]