疏水表面诱导稻瘟病菌侵染结构发育过程的基因表达谱分析  

Gene Expression Profile during the Development of Infecting Structures Induced by Hydrophobic Surfaces in Magnaporthe grisea

作　　者：骆红梅[1] 金庆超[2] 彭友良[3] 陈保善[4] 李有志[4] 邓烨[2] 戴承恩[2] 方永启[2] 董海涛[2] 李德葆[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036 [2]浙江大学生物技术研究所 [3]中国农业大学农业部分子植物病理学重点实验室,北京100094 [4]广西大学亚热带生物资源保护和利用实验室,南宁530004

出　　处：《农业生物技术学报》2007年第6期1034-1041,共8页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2002BA711A15)资助。

摘　　要：在制备完成高通量稻瘟病菌(Magnaphorthe grisea)cDNA阵列的基础上,平行检测孢子受疏水性表面诱导0、2、8、20和30h的基因表达谱。结果显示,在附着胞形成的不同阶段,均存在大量的差异表达基因,显示出这一过程中基因表达网络的复杂性。其中,附着胞在成熟阶段的差异表达基因数要明显高于其诱导和形成阶段,表现在基因表达谱20和30h时间点后发生剧烈变化。这种动态的基因表达谱的分析可以为稻瘟病菌致病的分子机理研究提供有意义的信息。Gene expression profiles were monitored at 0, 2, 8, 20, and 30 h time points of development of the infection structures induced by hydrophobic surfaces with a cDNA array with 4 108 TUTs from Magnaphorthe grisea. The results showed that there were many differentially expressed genes which indicated a complexity of gene expression network at any time-points of appresorium development. More genes were differentially expressed during appressorium maturation than during appressorium induction and formation. That is to say, genes seemed to be most actively expressed during 20 to 30 h time-points. Therefore, a detailed and comprehensive analysis of the programmed gene expression that occurs during the continuous stages of appressorium development is necessary to better understand the pathogenesis of M. grisea.

关 键 词：稻瘟病菌 附着胞 CDNA阵列 实时定量PCR 

分 类 号：S188[农业科学—农业基础科学]