背根神经节内P物质、降钙素基因相关肽免疫阳性神经元与阴茎包皮系带感觉信息的传递  

Substance P and/or Calcitonin Gene-related Peptide Immunoreactive Neurons in Dorsal Root Ganglia Possibly Involved in the Transmission of Nociception in Rat Penile Frenulum

作　　者：吴仲敏[1] 倪晶晶[2] 凌树才[3] 

机构地区：[1]台州学院医学院解剖学教研室,浙江台州318000 [2]宁波天一职业技术学院解剖学教研室,浙江宁波315100 [3]浙江大学医学院解剖学教研室,浙江杭州310031

出　　处：《中华男科学杂志》2007年第12期1068-1071,共4页National Journal of Andrology

基　　金：浙江省教育厅科研基金(20060077);台州市科技局科研基金(061ky42)

摘　　要：目的:探讨背根神经节(DRG)内P物质(SP)、降钙素基因相关肽(CGRP)免疫阳性神经元与阴茎包皮系带感觉信息传递之间的关系。方法:通过荧光金(FG)逆行标记对大鼠阴茎包皮系带内神经末梢的来源作追踪定位,并结合SP、CGRP免疫荧光标记法,研究大鼠DRG内FG标记阳性神经元中SP、CGRP免疫阳性神经元的形态和分布。结果:FG逆行标记结果发现,大鼠阴茎包皮系带内的神经末梢起源于第6腰髓对应的背根神经节(L6-DRG)和第1骶髓对应的背根神经节(S1-DRG)的神经元。对这些神经元分别作SP、CGRP免疫荧光标记后发现,标记细胞大小不等,分别呈深红色和深绿色,沿神经束成行排列或散在分布。FG/SP、FG/CGRP双标记阳性细胞均为中小型,其数量分别占FG逆行标记阳性细胞总数的1/3和1/2,FG/SP/CGRP三标记阳性细胞占FG逆行标记阳性细胞总数的1/5。结论:大鼠L6-DRG和S1-DRG内的SP、CGRP免疫阳性神经元可能参与阴茎包皮系带感觉信息的传递。Objective： To study the relationship between substance P （SP） and/or calcitonin gene-related peptide （CGRP） immuno- reactive neurons in dorsal root ganglia （DRG） and the transmission of nociception in the penile frenulum of rats. Methods ： The fluo- ro-gold（FG） retrograde tracing method was used to trace the origin of nerve terminals in the penile frenulum of rats. And SP and/or CGRP immunofluorescence labeling was employed to detect the distribution of SP and/or CGRP immunoreactive neurons in DRG. Reo suits： FG retrograde tracing showed that the FG retrolabeled neurons were localized in L6-DRG and S1-DRG. SP and/or CGRP immu- nofluorescence labeling indicated that a large number of DRG neurons were SP- and CGRP-immunoreactive, different in size, bright red and bright green respectively in color, and arranged in rows or spots among nerve bundles. All the FG/SP and FG/CGRP double-la- beled neurons were medium or small-sized. One third of the FG-labeled neurons were SP-immunoreactive, and a half of them CGRP- immunoreactive in L6-DRG and S1-DRG respectively. The FG/SP/CGRP-labeled neurons accounted for one fifth of the FG retro la-beled neurons. Conclusion： SP- and CGRP-immunoreaetive neurons in 1.6-DRG and S1-DRG of rats may be involved in the trans- mission of nociception in rat penile frenulum.

关 键 词：包皮系带 背根神经节 P物质 降钙素基因相关肽 荧光金 免疫组织化学 大鼠 

分 类 号：R33[医药卫生—人体生理学]