增生性瘢痕中基质金属蛋白酶及其抑制因子基因的表达  

Expression of matrix metalloproteinase-2,-9 and their inhibitor-1 in hypertrophic scars

作　　者：谢晓繁[1] 贺立新[1] 郝晓凤[2] 陈璧[2] 贾赤宇[2] 孙志刚[1] 曹玉珏[1] 李冬海[1] 

机构地区：[1]北京医院协会右安门医院烧伤整形科,100069 [2]第四军医大学西京医院全军烧伤中心

出　　处：《中华烧伤杂志》2007年第6期444-446,共3页Chinese Journal of Burns

摘　　要：目的了解基质金属蛋白酶2(MMP-2,又称明胶酶A)、MMP-9(又称明胶酶B)及其金属蛋白酶1组织抑制因子(TIMP-1)在增生性瘢痕不同形成时期的基因表达。方法提取16例人体不同时期增生性瘢痕样本和8例正常皮肤样本总RNA,分离mRNA,用反转录-聚合酶链反应法检测MMP-2、MMP-9和TIMP-1基因在不同样本中的表达。结果在正常皮肤中MMP-2、MMP-9和TIMP-1基因转录产物的灰度比值分别为(3.8±0.7)%、(5.8±4.4)%、(30.3±3.0)%,在增殖期瘢痕中分别为(13.5±4.5)%、(18.4±4.7)%、(37.7±4.3)%,明显高于正常皮肤(P<0.05)。成熟期瘢痕MMP-2和MMP-9基因表达量已恢复至正常水平,但TIMP-1基因较正常皮肤仍持续高表达(P<0.05)。结论MMP-2、MMP-9和TIMP-1基因表达增强可能是增生性瘢痕形成的机制之一,MMP-2和MMP-9基因表达降低可能与增生性瘢痕达到相对稳定的成熟状态有关。Objective To investigate the gene expression of matrix metalloproteinases （MMP-2, MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in proliferative and mature hypertrophic Methods Total RNA from 8 normal skin samples and from 16 human hypertrophic scar samples of different maturing stage was respectively extracted, and then mRNA was isolated. The gene expressions of MMP-2, MMP-9 and TIMP-1 in these samples were examined with reverse transcription-polymerase chain reaction （RT-PCR）. Results The gray scale ratio of MMP-2, MMP-9 and TIMP-1 transcription in normal skin were （ 3.8 ±0. 7 ） % , （ 5.8 ± 4.4） % , （ 30.3 ± 3.0 ） % , respectively, which were obviously higher than those in proliferative hypertrophic scar[ （ 14 ± 5 ） % , （ 18 ± 5 ） % , （ 38 ± 4） % , P 〈 0. 05 ]. The expression of MMP-2 and MMP-9 genes in mature hypotrophic scar returned to normal level, but that of TIMP-1 remained high when compared with that of normal level （ P 〈 0. 05 ）. Conclusion The increase in MMP-2, MMP-9 and TIMP-1 gene expression might be involved in the formation of hypertrophic scars, while the lowering of MMP-2 and MMP-9 gene expression might be associated with the maturation of hypertrophic

关 键 词：基质金属蛋白酶类 金属蛋白酶1组织抑制剂 瘢痕 肥大性 基因表达 

分 类 号：R622[医药卫生—整形外科]