磷脂酶A2抑制剂对腹膜透析大鼠腹膜通透性的影响  

The Effect of PLA_2 Inhibitor on the Peritoneal Permeability in the Dialyzed Rats

作　　者：黄胜[1] 李幼姬[2] 彭文兴[2] 李小艳[2] 阳晓[2] 

机构地区：[1]南方医科大学附属南海医院肾内科,佛山528200 [2]中山大学第一附属医院肾内科,广州510080

出　　处：《中国中西医结合肾病杂志》2007年第12期688-691,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：卫生部211工程重点科研基金资助项目(No98151)

摘　　要：目的:研究腹膜透析大鼠腹膜巨噬细胞磷脂酶A2(PLA2)活性及其抑制剂quinacrine对高糖透析大鼠腹腔液体超滤及溶质的影响。方法:(1)18只SD大鼠随机分成生理盐水组、1.5%葡萄糖组、4.25%葡萄糖组,透析10d后取腹膜巨噬细胞在96孔培养板培养24h后,用改良的Zieves法测定上清PLA2的活性;(2)将大鼠腹腔巨噬细胞分为正常对照组、30mmol/L甘露醇组、90mmol/L甘露醇组、30mmol/L葡萄糖组、90mmol/L葡萄糖组,每组6瓶。细胞裂解分别提取各自的蛋白后测定其蛋白总浓度;(3)12只SD大鼠随机分成4.25%葡萄糖透析组,4.25%葡萄糖+quinacrine(10mg/kg)透析组,透析10d后,准确量取腹腔内出水总量,分别测定透析液及血清同位素含量,葡萄糖及尿素氮浓度并进行腹膜动力学的计算。结果:生理盐水透析组、1.5%葡萄糖透析组、4.25%葡萄糖透析组各组巨噬细胞产生的PLA2的活性分别为13.5±1.56、45.0±3.34、101.0±6.52(P<0.05),高浓度葡萄糖能显著促进腹膜巨噬细胞PLA2蛋白合成(P<0.01)。4.25%葡萄糖+quinacrine透析组腹腔液体的超滤量、葡萄糖及尿素氮的转运均明显的得到改善(P<0.05)。结论:腹膜透析大鼠腹膜巨噬细胞PLA2的活性及蛋白表达随着透析中葡萄糖的浓度增加而升高,PLA2抑制剂quinacrine能明显改善腹膜透析大鼠腹膜的通透性。Objective：To study the synthesis of PLA2 in the cultured rat peritoneal macrophages under different glucose concentration and investigate the impact of PLA2 inhibitor on the ultrafiltration and solute transport properties. Methods： （ 1 ） 18 rats were randomly divided into three groups： When the rats were dialyzed for ten days , the peritoneal macrophages were cultured with DMEM/F12（ 10% FBS） for 24 hours, then the PLA2 activity were measured by the modified zieves way. （2）The macrophages acquired from the 15 Spradue Dawley rats were randomly divided into 5 groups （ n ： 6） and cultured under different glucose concentra- tion and different mannose concentration . The protein synthesis of PLA2 in the peritoneal macrophages was measured by Western blot, respectively. （3）Twelve male Sprague- Dawley rats were randomly divided into two groups including the group dialyzed by 4.25 % glucose（ n ： 6） and another dialyzed by 4.25 % adding PLA2 inhibitor （quinacrine, 10 mg/kg）Each rat from the two group received daily intraperitoneal injection （IP） of 25 ml dialysis solution for ten days Forty - eight hours after the last injection, a four hours dwell study with frequent dialysate and blood sampling was done, the peritoneal equilibrium test （PET） was carried. Results： The PLA2 activity in three groups treated by the normal water , 1.5% and 4.25% glucose dialysate were 13.5 ± 1.56,45.0 ± 3.34,101.0 ± 6.52 respective（ P 〈 0.05）. As compared to normal culture medium, 30 and 90 mM glucose can stimulate PLA2 protein synthesis in the rat peritoneal macrophages （P〈 0.01 ）, but 90 mM glucose stimulate more PLA2 protein synthesis than what 30 mM glucose does. Compared to the mannose culture medium, glucose stimulate more protein synthesis than the mannose does （P〈0.05）. the group treated by 4.25% glucose adding to the PLA2 inhibitor, the intraperitoneal absorption rate and solute transport rate were increased（P〈 0.05）. Conclusion： Conclusion： The glucose in

关 键 词：磷脂酶A2 腹膜透析 腹膜通透性 动物实验 

分 类 号：R692.5[医药卫生—泌尿科学]