尿转化生长因子-β_1和细胞外基质在慢性肾小球肾病中的变化  

Clinical Study of Urinary Tansforming Growth Factor-β_1 and Extracellular Matrix in Chronic Glomerulonephropathy

作　　者：周仁芳[1] 曾爱平[1] 李云生[1] 林曦[1] 柯颖杰[1] 陈瑛[1] 吴光华[1] 

机构地区：[1]温州医学院附属温岭医院,温岭317500

出　　处：《中国中西医结合肾病杂志》2007年第12期711-713,共3页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：温岭市科技局基金资助项目(No200646)

摘　　要：目的:探讨尿中转化生长因子-β1(TGF-β1)和细胞外基质(ECM)在各种慢性肾小球肾病患者中的变化及其临床意义。方法:将105例慢性肾小球患者进行临床和病理分组,采用酶联免疫吸附试验(ELISA)分别检测各组的尿TGF-β1水平,同时采用放射免疫分析法(RIA)检测尿中的各种ECM,所有检测值均用尿肌酐(Cr)浓度进行校正。结果:在不同临床分组中,尿TGF-β1/Cr和LN/Cr、PCⅢ/Cr、Ⅳ-C/Cr水平明显高于对照组(P<0.05),尿HA/Cr水平则在Ⅲ、Ⅳ组中出现下降。而在不同的病理分组中,肾小球轻微病变(GML)组尿中TGF-β1、ECM与对照组比较无统计学意义,其他各病理分组则出现不同的变化。相关性分析尿TGF-β1/Cr与LN/Cr、PCⅢ/Cr、Ⅳ-C/Cr正相关,与HA/Cr无相关性。结论:通过尿TGF-β1和ECM在慢性肾小球肾病患者中的联合检测,对评价慢性肾小球肾病的进展和预后判断提供一定的临床价值。Objective：To explore the changes and clinical significance of urinary transforming growth factor- β1 （TGF- β1 ） and extracellular matrix（ECM） in the patients with chronic glomerulonephropathy. Methods： 105 patients with chronic glomerulonephropathy were divided into groups by clinical manifestation or pathology, ELISA was used to measure urinary TGF - β1 level and RIA was used to determine urinary ECM level, All data were adjusted by urinary creatinine. Results. In different clinical groups, Urinary TGF- β1/Cr, LN/Cr, PCⅢ/Cr and Ⅳ - C/Cr were significantly higher than that of the control group（P〈0.05） ,but urinary HA/Cr level in Ⅲ and Ⅳ group were lower than control group , In different pathological groups,there were no significant difference in urinary TGF- β1 and ECM level between GML group and control group, however, the urinary TGF - β1 and ECM level were statistically different between other groups and control group. We also found urinary TGF-β1/Cr correlated positively with TGF - β1/Cr, LN/Cr, PCⅢ/Cr and Ⅳ - C/Cr, but did not correlated with HA/Cr. Conclusion： Combined examination of urinary TGF- β1 and ECM in parents with chronic glcmerulonephropathy can help to access progress and prognosis of chronic glomemlonephropathy.

关 键 词：肾小球肾病 转化生长因子-Β1 细胞外基质 

分 类 号：R692.6[医药卫生—泌尿科学]