高效液相色谱法测定葫芦素B人血清白蛋白纳米粒中药物含量  

Determination of cucurbitacin B in human serum albumin nanoparticles by HPLC

作　　者：王宁[1] 邓意辉[1] 王绍宁[1] 周欣羽[1] 赵静[1] 沈琳[1] 董晓辉[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国新药杂志》2007年第23期1968-1970,共3页Chinese Journal of New Drugs

摘　　要：目的：建立葫芦素B人血清白蛋白纳米粒中药物含量的测定方法。方法：采用Diamonsil C18柱（200mm×4.6 mm,5μm）;以乙腈-水-磷酸（45∶55∶0.1）作为流动相;流速为1 mL.min-1;检测波长为228 nm;柱温30℃。结果：在本实验条件下蛋白纳米粒中辅料对葫芦素B的测定无干扰,在1.0~20 mg.L-1浓度范围内线性关系良好（r=0.999 9,n=5）,葫芦素B平均回收率为100.2%,RSD为0.8%（n=3）。结论：本法准确可靠,简便易行,可用于葫芦素B人血清白蛋白纳米粒中药物含量的测定。Objective：To establish a HPLC method for the determination of cucurbitacin B in human serum albumin nanoparticles. Methods：The separation was performed on a Diamonsil C18 column （200 mm × 4.6 mm,5μm） at 30 ℃ , and the mobile phase was acetonitrile-water-phosphoric acid （45 ： 55 ： 0.1 ） , the flow rate was 1 mL· min^ -1 Cucurbitacin B was detected at 228 nm. Results： The linear range of cucurbitacin B was in the range of 1.0 - 20 mg· L^-1 （ r = 0. 999 9, n = 5 ）. The average recovery of cucurbitacin B was 100.2% , and RSD was 0.83% （n = 3）. Conclusion： An accurate and simple method was established for the determination of cucurbitacin B in human serum albumin nanoparticles.

关 键 词：葫芦素B 人血清白蛋白纳米粒 高效液相色谱法 含量测定 

分 类 号：R286.91[医药卫生—中药学] R927.2[医药卫生—中医学]