针灸对胃粘膜损伤的保护作用及其与NO的关系  

THE PROTECTIVE EFFECT OF ELECTROACUPUNCTURE AND MOXIBUSTION ON THE GASTRIC MUCOSAL DAMAGE AND THE RELATION WITH NITRIC OXIDE IN RATS

作　　者：裴文芬[1,2,3] 许冠荪 孙勇[1,2,3] 王月芳[1,2,3] 朱舜丽 张道芹[1,2,3] 

机构地区：[1]安徽中医学院生理教研室 [2]安徽中医学院针灸经络研究所 [3]浙江中医学院针推系

出　　处：《安徽中医学院学报》1997年第4期44-47,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：国家攀登计划

摘　　要：建立７０％乙醇灌胃致大鼠胃粘膜急性损伤模型，以胃粘膜血流量、胃跨壁电位差、胃粘膜损伤指数为指标，并测定血液中、胃粘膜中一氧化氮（ＮＯ）含量，研究针灸对胃粘膜损伤的保护作用及其与ＮＯ的关系。结果显示：针灸对胃粘膜损伤具有保护作用。ＮＯ的前体Ｌ精氨酸（Ｌａｒｇ）和ＮＯ的供体硝普钠（ＳＮＰ）对胃粘膜也有保护作用，而ＮＯ合成酶的抑制剂Ｎ亚硝基左旋精氨酸（ＬＮＮＡ）未显示对胃粘膜有保护作用。Ｌａｒｇ和ＳＮＰ预处理后，可加强针灸对胃粘膜的保护作用，而ＬＮＮＡ则有减弱针灸对胃粘膜的保护作用，用ＬＮＮＡ及Ｌａｒｇ同时预处理，则又增强了针灸对胃粘膜保护作用。结论：针灸对胃粘膜的保护作用可能通过ＬａｒｇＮＯ通路实现的。Acute model of gastric mucosal damage induced by perfusion of the 70% ethanol to stomach was established.Gastric mucosal blood flow,transmucosal potential difference and gastric mucosal lesion index as indexes and the contents of nitric oxide (NO) in blood and gastric mucosal were detected.The protective effect of electroacupuncture(EA) and moxibustion(M) on the gastric mucosal damage in rats and the relation with nitric oxide were studied.The results obtained were as follows:EA and M may show the protective effect on the gastric mucosal damage in rats.Both precursor of NO L arginine(L arg) and donor of NO natrii Nitroprussidum(SNP) may also show the protective effect on the gastric mucosal damage in rats as well.But NO synthesis inhibitor N w nitro L arginine(L NNA) could not show the protective effect of the gastric mucosal damage in rats.Both by pretreament with L arg and SNP,the protective effectof EA and M on the gastric mucosal damage in rats were increased,but by pretreament with L NNA and L arg,the protective effect of EA and M on the gastric mucosal damage were reversed.Conclusion:the protective effect of EA and Mon the gastric mucosal damages in rats was mediated through actvation of L arg NO pathway.

关 键 词：一氧化氮 胃粘膜损伤 针灸疗法 

分 类 号：R259.73[医药卫生—中西医结合] R246.1[医药卫生—中医内科学]