重组人骨形成蛋白2和转化生长因子β复合促进骨生长  

Experimenial study on the enhancement of bone formation by recombinant human BMP and TGFβ

作　　者：司晓辉[1] 金岩[1] 杨连甲[1] 

机构地区：[1]西安第四军医大学口腔医学院病理科

出　　处：《实用口腔医学杂志》1997年第3期166-168,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金

摘　　要：将重组人骨形成蛋白２（ｒｈＢＭＰ２）和转化生长因子β（ＴＧＦβ）复合后，对比观察其骨诱导及骨生成过程和促进骨生长的效果。以一定比例的ＴＧＦβ和ｒｈＢＭＰ２与陶瓷化牛骨（ＣＢＢ）复合，埋入小鼠股部肌肉陷窝内，于第３、５、７、１４、２１ｄ取材后光镜观察新骨生长情况并计算新骨形成面积。结果发现，ｒｈＢＭＰ２和ＴＧＦβ复合后其成骨作用比单独应用ｒｈＢＭＰ２明显，骨组织较为成熟，新生骨面积１４ｄ时２倍于ｒｈＢＭＰ２组。而单独用ＴＧＦβ无异位诱导新骨形成的作用，但ＴＧＦβ可刺激原有骨的膜内成骨。认为，ＢＭＰ和ＴＧＦβ在骨生长修复中各自发挥着不同的作用，ＴＧＦβ可促进ＢＭＰ的骨诱导过程，ＢＭＰ／ＴＧＦβ／ＣＢＢ复合物或ＢＭＰ／ＴＧＦβ／生物陶瓷复合物具有高效的骨诱导性，在临床骨缺损及骨折修复中有良好的应用前景。To observe the ectopic bone formation, the complexes of recombinant human BMP2(rhBMP2)/CBB(ceramic bovine bone), TGBβ/CBB, rhBMP2/TGFβ/CBB were prepared and implanted into the thigh muscle pouches of mice respectively. The histological reactions were observed at the intervals of 3,5,7,14 and 21 days after the implantation. The combination of rhBMP2 and TGFβ showed stronger effects in the stimulation of bone formation than rhBMP alone. The quantity of the new bone produced by rhBMP2/TGFβ/CBB was twice as much as that of rhBMP/CBB. TGFβ alone did not induce the ectopic new bone formation, but initiated femoral intramembranous bone formation. It is concluded that: (1) The combination of BMP and TGFβ can not only induce new bone formatin, but also promote the mature of skeletal tisues; (2) TGFβ is the one which is able to influence and promote the process of BMP bone induction;(3) The complex of BMP/TGFβ/CBB or BMP/TGF β/bioceramics may be a high efficient bone inducing material in clinical bone defect repair in the future.

关 键 词：人骨形成蛋白 转化生长因子 骨生长 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]