颌面部枪弹伤软组织内一氧化氮含量与组织细胞损伤  

Nitric oxide and soft tissue injurg in maxillofacial region after gunshot wound

作　　者：朱哓天[1,2,3] 周树夏[1,2,3] 刘荫秋 李曙光[1,2,3] 王多宁 

机构地区：[1]第四军医大学口腔颌面外科 [2]第三军医大学野战外科研究所六室 [3]第四军医大学化学教研室

出　　处：《实用口腔医学杂志》1997年第3期175-177,共3页Journal of Practical Stomatology

摘　　要：在Ｄ５．８０ｍｍ制式枪弹致伤动物模型上的颌面部，于致伤后２、６、２４、７２ｈ、７ｄ取材，检测组织内ＮＯ的含量，并用ＨＥ和ＮＡＤＰＨ－ｄ染色观察组织形态学变化与一氧化氮合酶（ＮＯＳ）活性的关系。结果发现：伤后组织内ＮＯ含量明显增高，２４ｈ达到高峰，７ｄ维持较低水平；２４～７２ｈ组织呈进行性损伤，组织内可见大量ＮＯＳ阳性细胞，７ｄ组织内修复现象明显，ＮＯＳ阳性细胞明显减少。结果提示：颌面部枪弹伤后组织进行性损伤和修复愈合与Ｌ－精氨酸／ＮＯ途径的激活有关；ＮＯ的大量生成是组织细胞损伤的重要因素之一。Gunshot wound was created by 5.80mm bullet in maxillofacial region of dogs. Samples were harvested at each timepoint of 2h, 6h, 24h, 72h and 7d after wound. Nitric oxide(NO) content in the samples was detected. The relationslip between histopathological changes and NO synthase(NOS) were observed dynamically with HE and NADPH d stain. It was found that NO content was increased and reached peak 24h after wound and maintained a low level in 7d. Tissue damage was more serious in 24～72h. There were many NOS positive cells in wounded tissue. 7d after wound, NO content was decreased significantly. The results suggested that there was certain relationship between L Arg/NO pathway and tissue damage. It seems that large amount of NO may be one of the important reasons which cause local tissue injury in the gunshot wound.

关 键 词：颌面部 枪弹伤 一氧化氮 组织细胞损伤 

分 类 号：R826.502[医药卫生—临床医学] R782.402