金钱树组培快繁技术研究  

Study on Rapid Vitro Propagation of Zamioculcas Zamiifolia

作　　者：潘学峰[1] 王俊豪[1] 符青苗[1] 

机构地区：[1]海南大学农学院,海南海口570228

出　　处：《海南大学学报（自然科学版）》2007年第4期402-407,共6页Natural Science Journal of Hainan University

摘　　要：以金钱树叶片为外植体进行离体培养研究,结果表明:用ρ=0.1 g.L-1的升汞溶液对金钱树叶片消毒12 m in,效果较佳;在外植体愈伤组织诱导中,成熟叶较嫩叶易产生愈伤组织;1 200 lx的光强有利于愈伤组织的形成;2,4-D1.0 mg.L-1+6-BA2.0 mg.L-1的激素配比对诱导叶片愈伤组织的形成有利,诱导率高达92.0%;在所试的3种基本培养基中,H基本培养基较适宜愈伤组织的分化;6-BA8.0 mg.L-1对愈伤组织分化不定芽的效果较好,不定芽在1/2MS+NAA1.0 mg.L-1的生根培养基上,生根率高达100%,根多,且壮,植株长势好;试管苗移栽在河沙及表土混成(V河沙∶V表土=1∶1)的基质中,其成活率较高,可达86.2%.The leaves of Zamioculcas zamiifolia Engl. were used as ex-plants in vitro culture. The results in this experiment showed that the leaf 12 min treated with 0.1% HgCl2 had a good sterilization. Under the inducing callus of ex-plants, mature leaf produced callus is more easy than young leaf. 1200 lx light intensity was good for the formation of the callus. 2,4 -D 1.0 mg · L^-1 ＋6 -BA 2.0 mg · L^-1was good for the formation of the callus, the callus inducing rate could reach as high as 92.0%. Among three tested mediums, H medium was suit for callus differentiation. The optimum concentration of 6 - BA was 6 - BA 8.0 mg · L^-1 for adventitious bud forming from the callus. In rooting medium with 1/2MS ＋ NAA 1.0 mg · L^-1 , the rooting rate found was 100% , that the plants have more roots, and stronger, and good growth potent. When transplanted to the matrix composed of 1 ： 1 river sand and surface soil, tube plants have a survive rate as high as 86.2%.

关 键 词：金钱树 叶片 愈伤组织 组培快繁 

分 类 号：S336[农业科学—作物遗传育种]