大鼠急性心肌梗死后ACE、ACE2基因异常表达的意义及福辛普利干预的影响  

Significance of Abnormal Expression of ACE and ACE2 Gene in Rats After AMI and the Effect of Fosinopril on ACE and ACE2

作　　者：陈高[1] 黄从新[2] 赵庆彦[2] 陈辉霖[1] 

机构地区：[1]武汉大学人民医院,核医学科430060 [2]武汉大学人民医院心内科,湖北武汉430060

出　　处：《实用临床医药杂志》2007年第6期8-11,共4页Journal of Clinical Medicine in Practice

基　　金：武汉大学十五211工程建设项目(20031684)

摘　　要：目的研究急性心肌梗死(AMI)后左室梗死区及非梗死区ACE、ACE2 mRNA的表达以及福辛普利对ACE、ACE2 mRNA表达的影响。方法结扎冠状动脉前降支诱导大鼠急性心肌梗死,AMI后24 h存活的24只大鼠随机分为AMI组、福辛普利组,每组8只。另设8只假手术组;术后24 h开始直接灌胃给药,AMI及假手术组大鼠灌等量生理盐水;用药28d后,用RT-PCR法检测左心室梗死区及非梗死区心肌ACE和ACE2 mRNA表达的情况。结果大鼠AMI 28 d后左心室梗死区及非梗死区心肌ACE、ACE2 mRNA表达均较假手术组明显升高(P<0.01),福辛普利组梗死区及非梗死区ACE mRNA表达较心梗组均显著降低(P<0.05),但仍高于假手术组(P<0.05);福辛普利对ACE2 mRNA的表达几乎无影响,心梗组及福辛普利组梗死区及非梗死区ACE2 mRNA表达无明显差异(P>0.05)。结论AMI后ACE2 mRNA表达增多在心肌损伤后肾素血管紧张素系统有关的血管紧张素肽的产生与降解的负性调节中起着重要的作用。Objective To study the changes of expression of ACE and ACE2 at mRNA level in infarction and non-infarction zone of left ventricule and to evaluate the effect of fosinopril on ACE and ACE2 expression after acute myocardial infarction（AMI） in rats. Methods AMI was established by ligation of the anterior descending coronary artery in female Spague-Dawly （SD） rats. Twenty-four hours after the procedure, the 24 surviving rats were grouped randomly as AMI group （ n = 8）, fosinopfil treatmen tgroup （ n = 8）, Sham operated rats were selected randomly to serve as non infarcted controls （n = 8）. 4 weeks after treatment with the drugs by gastric gavage, the relative expression of myocardium ACE and ACE2 mRNA was assessed by reverse transcription-polymerase chain reaction （RT-PCR）. Results The mRNA expression of ACE and ACE2 in AMI group were significantly elevated compared with sham group （P〈0.01）. The mRNA expression of ACE in fosinopril group was markedly attenuated compared with AMI group （P 〈 0.05）, but increased compared with sham group （P 〈 0.05）. In contrast, fosinopril had no effect on cardiac ACE2 mRNA, which remained elevated at the same levels in all areas of the AMI rat heart （P〉0. 05）. Conclusion The increase in ACE2 after AMI suggests that it plays an important role in the negative modulation of the renin angiotensin system in the generation and degradation of angiotensin peptides after cardiac injury.

关 键 词：大鼠 ACE2 MRNA 心肌梗死 福辛普利 

分 类 号：R542.2[医药卫生—心血管疾病]