BADH引物检测水稻香味基因的研究  

Determining Aroma Genes in Rice by Using BADH Premier and Its Application in the Breeding of Fragrant Rice

作　　者：杨占烈[1] 

机构地区：[1]贵州省农业科学院水稻研究所,贵州贵阳550006

出　　处：《安徽农业科学》2007年第35期11390-11391,11393,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了检测水稻中的香味基因,为其在香稻选育中的应用提供依据。[方法]从泰国香稻的回交后代材料BC3F2和中国的香稻中提取DNA后,加入BADH引物进行PCR扩增,并进行聚丙烯酰胺凝胶电泳检测香味基因。同时,对含香味基因的单株利用KOH溶液作叶片香味鉴定,并比较两种检测结果。[结果]电泳结果表明,94份样品中有32份样品含有纯合的香味基因,19个样品为香味基因的杂合体,与KOH溶液法检测香味的结果一致。BADH引物PCR扩增结果表明,香味来源于新香B的后代材料能产生明显的DNA扩增带,其香味基因与KDLM105以及Basmati香稻品种的香味基因均位于第8染色体上,属于等位基因。[结论]在香稻品种选育中,利用BADH引物能准确检测出含香味基因的植株,显著地缩短香稻品种育成的年限。Objective The research aimed to determine the aroma genes in rice and provide basis for its application in the breeding of fragrant rice.Method After DNA was extracted from backcross progeny materials BC3F2 of Thailand fragrant rice and Chinese fragrant rice,BADH premier was added for PCR amplification and determine the aroma genes by polyacrylamide gel electrophoresis.At the same time,aroma of leaf blade was identified on the individual plants contained aroma genes by using KOH solution.Two kinds of the detection results were compared.Result The electrophoresis results showed that among 94 samples,32 samples contained homozygous aroma genes and 19 samples were heterozygotes of aroma genes,which was accordant with the results of determining aroma by KOH solution method.The PCR amplification results of BADH premier showed that the progeny materials that aroma rooted in Xinxiang B could produce obvious DNA amplification bands,whose aroma genes and the aroma genes in KDLM105 and Basmati fragrant rice varieties all lay on chromosome 8 and belonged to alleles.Conclusion In the breeding of fragrant rice varieties,using BADH premier could determine the plants contained aroma genes exactly and shorten the breeding period of fragrant rice varieties significantly.

关 键 词：BADH 检测 水稻香味基因 香稻选育 应用 

分 类 号：Q78[生物学—分子生物学]