白芍总甙对大鼠腹腔巨噬细胞Ca^(2+)跨膜转运的影响  

Effect of total glucosides of paeony on calcium ion transport across membrane of peritoneal macrophages in rats

作　　者：王斌[1] 陈敏珠[1] 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理学研究所

出　　处：《中国药理学与毒理学杂志》1997年第3期215-217,共3页Chinese Journal of Pharmacology and Toxicology

摘　　要：通过检测大鼠腹腔巨噬细胞（Ｍ）胞浆游离钙离子浓度（［Ｃａ２＋］ｉ）和４５Ｃａ内流与外排研究了不同浓度白芍总甙（ＴＧＰ）对ＭＣａ２＋跨膜转运的影响．结果表明，胞外有Ｃａ２＋时，ＴＧＰ（２．５－２０ｍｇＬ－１）对亚适浓度卡西霉素（０．１μｍｏｌＬ－１），脂多糖（ＬＰＳ，５ｍｇＬ－１）和甲酰三肽（ｆＭＬＰ，１μｍｏｌＬ－１）升高Ｍ的［Ｃａ２＋］ｉ具有明显的增强作用；而胞外无Ｃａ２＋时，ＴＧＰ对ＬＰＳ或ｆＭＬＰ刺激胞内钙池释放Ｃａ２＋引起［Ｃａ２＋］ｉ升高的作用无明显影响；ＴＧＰ可使卡西霉素刺激Ｍ的４５Ｃａ内流明显增加，同时较高浓度（１０－４０ｍｇＬ－１）亦浓度依赖性促进４５Ｃａ外排．这些结果提示，ＴＧＰ有促进胞外钙内流作用，较高浓度ＴＧＰ可促进胞内钙外排，从而降低［Ｃａ２＋］ｉ．Effect of different concentrations of total glucosides of paeony (TGP) on calcium ion across membrane of rat peritoneal macrophages (PM ) were studied by measuring cytoplasmic free Ca 2+ concentration ([Ca 2+ ] i) and 45 Ca fluxes in PM  from rats. The results showed that TGP (2.5-20 mg·L 1 ) had a marked enhancement on elevated [Ca 2+ ] i in PM  stimulated by the suboptimal concentrations of calcimycin(0.1 μmol·L 1 ), lipopolysaccharide (LPS, 5 mg·L 1 ) or formyl methionine leucine phenylalanine (fMLP, 1 μmol·L 1 ), in the presence of extracellular Ca 2+ (1 mmol·L 1 ), but TGP did not have any effect on increased [Ca 2+ ] i through releasing Ca 2+ in its internal reservoir of PM  induced by LPS or fMLP. TGP could increase 45 Ca influx, but its higher concentrations (10-40 mg·L 1 ) could promote 45 Ca efflux. These results suggest that TGP can increase the influx of extracellular calcium, and that the higher concentration of TGP can promote efflux of intracellular calcium and then down reglate the intracellular calcium concentration.

关 键 词：白芍总甙 巨噬细胞 白芍 跨膜转运 钙 

分 类 号：R285.5[医药卫生—中药学]