水稻种胚LOX3基因在逆境胁迫中的作用  

Role of Embryo LOX3 Gene under Adversity Stress in Rice(Oryza sativa)

作　　者：刘南南[1] 江玲[1] 张文伟[1] 刘玲珑[1] 翟虎渠[2] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏南京210095 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国水稻科学》2008年第1期8-14,共7页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2004CB117204,2006CB100200);农业结构调整重大专项资助项目(05-01-05B);长江学者和创新团队发展计划资助项目

摘　　要：LOX3是主要的水稻种胚脂氧合酶同工酶。为了研究水稻LOX3基因在逆境胁迫中的作用,构建了LOX3基因的反义植物表达载体,用农杆菌介导法转化水稻品种武运粳7号和Kasalath,获得了转基因植株。PCR和Southern鉴定证实基因已经导入水稻基因组中,种胚LOX3缺失鉴定和半定量RT-PCR分析证实反义RNA抑制了LOX3基因的表达。对T2代转基因植株进行了水分胁迫处理及稻瘟病和白叶枯病的接种鉴定。结果显示,与非转基因对照相比,反义LOX3转基因植株对水分胁迫、稻瘟病和白叶枯病都表现敏感,说明水稻种胚LOX3基因在逆境胁迫反应中发挥一定的作用。Lipoxygenase 3 （LOX3） is the major component among the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under adversity stress, plant expression vector containing antisense of embryo LOX3 eDNA was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. The expression of LOX3 gene was suppressed in transgenic plants with antisense gene, which was confirmed by analyzing of embryo LOX3 deletion and semi-quantitive RT PCR. When T2 transgenic plants were subjected to drought stress and infected with rice blast and bacterial blight, the transgenic plants with the suppression of LOX3 gene were sensitive to drought stress, rice blast and bacterial blight compared with non transgenic plants. These results suggested that embryo LOX3 gene had some functions in response to adversity stress.

关 键 词：水稻 脂氧合酶基因 反义RNA 转基因植株 逆境胁迫 

分 类 号：S511[农业科学—作物学]