水稻细菌性谷枯病病原菌的分离鉴定  

Isolation and Identification of the Causal Organism of Bacterial Grain Rot from Rice

作　　者：罗金燕[1] 徐福寿[2] 王平[3] 徐丽慧[1] 谢关林[1] 

机构地区：[1]浙江大学生物技术研究所,浙江杭州310029 [2]杭州市植物保护土肥总站,浙江杭州310020 [3]黑龙江省农垦科学院,黑龙江佳木斯154007

出　　处：《中国水稻科学》2008年第1期82-86,共5页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30671397)

摘　　要：经多年的病原菌分离监测,于2006年从2份浙江省稻种(来自海南的健康籼稻)上分离到6株病原细菌,经25项主要生理生化特性、菌落形态、致病性、Biolog、脂肪酸分析和RAPD-PCR鉴定,发现这种病原细菌为Burkholderia glumae,是引起水稻细菌性谷枯病的病原菌。此外,实验证明"健康稻种"也可能存在水稻细菌性谷枯病菌。A survey on isolation and detection of the casual organism of bacterial grain rot from rice was conducted during 1997 2006. In 2006, six pathogenic bacterial strains were isolated from two asymptomatic rice seed samples （Oryza sativa L. ） originally produced in Hainan Province and then planted in Zhejiang Province. They were identified as Burkholderia glumae, which is the causal organism of bacterial grain rot of rice, by physiological characteristics, colony morphology, pathogenicity test, Biolog, fatty acid methyl ester （FAME） analysis and RAPD-PCR comparcd with the four standard reference strains. It is confirmed that there exsits Burkholderia glumae in so-called ＂health looking seeds＂.

关 键 词：水稻细菌性谷枯病菌 表型鉴定 分子鉴定 致病性 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]