X线反复照射人肺癌细胞系Anip973后的生物特性变化  

Effect of repeated irradiation on biological characteristics of lung adenocarcinoma cell line Anip973 in vitro

作　　者：许庆勇[1] 徐向英[1] 杨伟志[2] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院放疗科,哈尔滨150081 [2]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所放疗科放射生物室,北京100021

出　　处：《中华放射肿瘤学杂志》2008年第1期51-54,共4页Chinese Journal of Radiation Oncology

基　　金：黑龙江省青年科学技术专项资金（QC06C067）;哈尔滨市青年科学研究基金（2005AFQXJ065）

摘　　要：目的通过X线反复照射体外培养的肺腺癌细胞系Anip973，初步探寻其生物学特征的变化。方法应用高能X线反复照射肺腺癌Anip973细胞15次，4Gy/次，共60Gy。对亲代和子代细胞采用克隆形成实验测定其放射敏感性。根据生长曲线计算Anip973R细胞及其亲代细胞倍增时间，用流式细胞术检测细胞周期分布特征。结果与亲代相比Anip973R细胞D0、Dq、SF2均增大，d值减小，分别为1．61、1．29、0．532、0．312，而Anip973细胞分别为1．53、0．42、0．339、0．3524。Anip973R细胞放射耐受性增高，其细胞倍增时间较亲代细胞延长3h。细胞周期显示G1、S期比例分别增高、减少（P〈0．05），G2＋M期比例相似（P〉0．05）。结论Anip973细胞经X线反复照射后与亲代细胞相比放射耐受性增高、细胞倍增时间延长。Objective To study the effect of repeated irradiation on biological characteristics of human lung adenocarcinoma cell line Anip973 in vitro. Methods Anip973 cells were treated with high energy X-ray to a total dose of 60 Gy at 4 Gy fractions. The radiosensitivity of Anip973R and its parental cell were measured by clonogenic assay. The biological parameters were fitted to the single hit multitarget formula. Furthermore, the population double time（PDT） and cell cycle distribution were measured by cell growth curve and flow cytometry, respectively. Results Comparing with its parental cell, Anip973R acquired radioresistance showing increased Do, Dq and SF2 and a broader shoulder. PDT of Anip973R extended 3 h more than that of Anip973. The Anip973R also showed higher and lower percentage of cells in Gl and S phase （ P 〈 0. 05 ）, respectively, but similar GJM distribution（ P 〉 0. 05 ）. Conclusions A radioresistant lung adenocarcinoma cell line Anip973R is established by repeatedly irradiation. Its radioresistance displays obviously in lower dose area. However, its characteristic of cell cycle is not completely coincident with the classical radiobiological theory.

关 键 词：肺腺癌细胞系 人 分次照射 辐射耐受 

分 类 号：R686[医药卫生—骨科学]