黄土高原不同气象因子对中国沙棘亚居群遗传多样性的影响  

Genetic diversity of Hippophae rhamnoides Lsub-populations on Loess Plateau under effects of varying meteorologic conditions

作　　者：杨明博[1] 熊友才[1] 王红芳[1] 巫晓华[1] 薛泽冰[1] 牟硕[1] 刘志才[1] 葛剑平[1] 

机构地区：[1]北京师范大学生命科学学院生物多样性与生态工程教育部重点实验室,北京100875

出　　处：《应用生态学报》2008年第1期1-7,共7页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(2002CB111507);国家"十一五"科技支撑计划资助项目(2006BAD03A0206).

摘　　要：利用ISSR分子标记技术,分析了甘肃省东部地区8个中国沙棘居群的遗传多样性和遗传分化,以及气象因子对它们的影响.共调查了8个居群的240个个体,利用11条引物扩增出165条带.扩增范围集中在300～1500bp,其中157个位点表现为多态,占95.76%,分子变异分析(AMOVA)显示,居群内遗传分化程度高,76.5%的变异存在于居群内.基因分化系数(Gst)为0.2418,居群间基因流为1.5675.说明保护沙棘种群的遗传资源应优先考虑种群内个体.经Mantel检验发现,8个居群的地理距离和遗传距离显著相关(r=0.65,P=0.002).对气象因子与遗传多样性所作的回归分析显示,中国沙棘开花期间的风速与其遗传多样性呈显著正相关.说明开花期风速和地理距离是沙棘种群遗传多样性的重要影响因子.The genetic diversity and genetic differentiation of eight Hippophae rhamnoides L. populations in eastern Gansu Province, as well as the effects of varying meteorologic conditions on them were assessed by using inter-simple sequence repeat （ISSR） molecular marker method. A total of 240 individuals in the populations were sampled. Using eleven primers, 165 bands were generated, ranging in size from 300 to 1 500 bp, and 157（95.76% ） were found to be polymorphic. The analysis of molecular variance （AMOVA） demonstrated that there was a relatively high level （76.5%） of genetic variation within the populations, with the gene differentiation coefficient （Gst） and gene flow being 0. 2418 and 1. 5675, respectively. Therefore, to protect the gene resources of H. rhamnoides, the individuals within the populations should be first considered. Mantel test showed that genetic distance was significantly positively correlated with geographical distance （ r = 0.65, p = 0. 002）, and regression modeling between genetic diversity and meteorologic factors suggested that there was a significant positive correlation between wind speed during blooming and genetic diversity of H. rhamnoides, illustrating that wind speed in blooming period and geographic distance were the vital factors affecting the genetic diversity of H. rhamnoides population.

关 键 词：中国沙棘 气象因子 遗传多样性 ISSR标记 

分 类 号：Q943[生物学—植物学]