桔梗花药培养初报  

Preliminary Study of Anther Culture of Platycodon grandiflorum

作　　者：吴京姬[1] 吴基日[1] 吴松权[1] 严一字[1] 王立平[1] 

机构地区：[1]延边大学农学院,龙井133400

出　　处：《植物研究》2008年第1期79-81,共3页Bulletin of Botanical Research

基　　金：吉林省科技厅基础处基金项目(20040553)

摘　　要：把花粉发育至单核靠边期的桔梗花药接在6种不同组合的培养基上诱导愈伤组织,并把诱导的愈伤组织转接到分化培养基,试图得出单倍体植株。结果表明:诱导率最高的培养基组合是N6+2,4-D 0.2 mg.L-1+6-BA1.0 mg.L-1,诱导率为89.6%;在N6+6-BA1.0 mg.L-1+NAA0.5mg.L-1的分化培养基上最高分化率可达58.4%;经根尖压片检查染色体数目结果,分化的绿苗中弱小的植株是单倍体植株。Pollen of Platycodon grandiflorum growing to monokaryotic stage was inoculated in six different culture medium and induced callus, the induced callus was transfered in differentiation culture, trying to get haploidy. The results showed that the culture medium of the highest induced rate of callus was N6 ＋2,4-D 0. 2 mg·L^-1 ＋6-BA 1.0 mg·L^-1 and induced rate was 89.6%, the highest differentiation rate was 58.4% in the culture of N6 ＋6-BA1.0 mg·L^-1 ＋ NAA0. 5 mg·L^-1 , puny green plant in differentiation was haploidy with the result of chromosome number of root.

关 键 词：桔梗 花药培养 单倍体植株 

分 类 号：Q943[生物学—植物学]