酶促微粒体脂质过氧化模型的建立  

Foundation of enzymatic microsomal lipid peroxidation model

作　　者：孙贵范[1] 李富君[1] 邱立岩[1] 丁桂英[1] 

机构地区：[1]中国医科大学预防医学系劳动卫生教研室

出　　处：《中国地方病学杂志》1997年第4期197-199,共3页Chinese Jouranl of Endemiology

摘　　要：动用全外实验，对ＮＡＤＰＨ／ＦｅＳＯ４诱发的微粒体脂质过氧化（ＬＰ）发生条件及反应历程进行了探讨。结果表明：在３２７℃水浴，微粒体蛋白浓度为１．０ｍｇ／ｍｌ，ｐＨ值７．４的０．１ｍｏｌ／ＬＴｒｉｓ－ＨＣＬ缓冲液的条件下，１５０μｍｏｌ／ＬＮＡＤＰＨ和４．４５μｍｏｌ／ｌ／ＬＦｅＳＯ４可使微粒全中丙二醛（ＭＤＡ）含量于反应１５分钟内迅速上升，３０分钟时达到高峰，以后趋于平稳。揭示一定比例的ＮＡＤＰＨ／ＦｅＳＯ４可有效地激发微粒体ＬＰ。The condition and the time coures of lipid peroxidation(LP)induced by NADPH/FFeSO 4 in rat hepatic microsomes in vitro were studied.The result revealed that malonaldehyde(MDA)content in microsomes rapidly increased within 15 minutes after addition of 150 μmol/L NADPH and 4.45μmol/L FeSO 4,reached maximal level at 30 minutes,and became constant over the next one hour,when microsomes were incubated at 37℃ in 0.1μmol/L Tis-HCI buffer C=1.0mgPr/ml.pH=7.47.This result suggested that appropiate scale of NADPH/FeSO 4 could effectively induce microsomal liopid peroxidation.A model of enzymatic lipid peroxidation was preliminarily founded in our experiment.

关 键 词：还原型辅酶Ⅱ 微粒体模型 脂质过氧化 

分 类 号：Q552[生物学—生物化学]