SP1在增生性瘢痕组织中表达的研究  

Study of SP1 Expression in Hypertrophic Scar

作　　者：郑江红[1] 万伟东[2] 邓辰亮[1] 高云[1] 张世新[1] 杨松林[1] 

机构地区：[1]上海市上海交通大学附属第六人民医院,200233 [2]江苏省南京市东南大学附属中大医院,210009

出　　处：《组织工程与重建外科杂志》2007年第6期321-323,共3页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(30571929)

摘　　要：目的通过比较人增生性瘢痕与正常皮肤组织基因转录SP1表达水平,研究增生性瘢痕中SP1表达所发生的改变,并探讨其意义。方法收集增生性瘢痕组织及正常皮肤组织,应用荧光定量PCR技术检测SP1mRNA在正常皮肤和增生性瘢痕组织中的表达。结果样品与内参照物基因组拷贝数的比值为(SP1/GAPDH×100%),增生性瘢痕组为(252.66±145.91)%,正常皮肤组为(3.90±3.45)%,相差具有统计学意义(p<0.05)。结论应用荧光定量PCR方法可对细胞内SP1进行较准确的测定,方法简便且敏感。增生性瘢痕组织中SP1的高表达可能是瘢痕增生的重要因素。Objective To investigate the changes and significance of SP1 expression based on the comparison of SP1 expression level in hypertrophic scar and normal skin tissue. Methods After collecting hypertrophic scar and normal skin tissue, SP1 mRNA expression in hypertrophic scar by real time-PCR was detected. Results The ratio of SP1 and GAPDH in hypertrophic scar sample group is （252.66±145.91） %, while（3.90±3.45） % in normal skin group. The difference between the two groups is statistically significant. Conclusion Real time-PCR is an accurate and sensitive method for SP1 mRNA measurement. Higher expression of SP1 mRNA in hypertrophic scar tissue may be an important factor in scarring.

关 键 词：增生性瘢痕 转录因子 基因 

分 类 号：Q786[生物学—分子生物学]