竹根七中具有血管生成抑制作用有效成分的筛选  

Screening the Effective Component of Rhizoma Tupistrae Chinensis with Inhibiting Angiogenesis

作　　者：蒙麦侠[1] 杨智锋[1] 贺浪冲[2] 

机构地区：[1]陕西中医药研究院,陕西西安710003 [2]西安交通大学药学院,陕西西安710061

出　　处：《中国中医药信息杂志》2008年第1期46-47,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的研究竹根七中对人脐静脉内皮细胞株(ECV304)生长有抑制作用的有效成分。方法用溶剂提取法将竹根七分为不同药用部位,以抗血管内皮细胞生长因子(VEGF)抗体为对照,用人脐静脉内皮细胞膜色谱模型筛选,并对筛选结果用MTT实验进行分析,以初步确定其中的有效成分。结果竹根七的分离成分(ZGQ-C)在其浓度分别为0.625、1.25、2.50、5.00、10.0μg/mL时,对ECV304的增殖抑制率分别为40.51%、51.11%、63.54%、74.32%、81.26%,半数抑制率在1.00～1.25μg/mL范围内;同时在显微镜下观察到细胞的形态有明显的改变。结论ZGQ-C是对ECV304有一定抑制作用的有效成分;人脐静脉内皮细胞膜色谱模型的筛选结果与MTT实验结果之间有较好的相关性。Objective To discover the effective component of Rhizoma Tupistrae chinensis with inhibiting angiogenesis. Methods Several parts of Rhizoma Tupistrae chinensis were obtained by using different extraction solvents. They were screened by the model of ECV304 cell membrane chromatography with anti-VEGF antibody as a control drug. The inhibition of effective component （ZGQ-C） on ECV304 proliferation in vitro was examined by MTT assay, Results The inhibition rate （40.51%, 51.11%, 63.54%, 74.32%, 81.26%） was correlated with the ZGQ-C concentration. The ECV304 cell had significantly changed in morphology. Conclusion The ZGQ-C is an effective component for inhibiting angiogenesis. The screening results on the model have a good correlation with that of MTT assay.

关 键 词：竹根七 细胞膜色谱 ECV304 抑制血管生成 

分 类 号：R284.2[医药卫生—中药学]