三七总皂甙对传代肝星状细胞增殖的影响  

Effect of PNS on proliferation of HSC-T6 cells

作　　者：王怡[1] 薛绍礼[1] 张菁[2] 薛青[3] 汪亚松[1] 夏觅真[2] 孔云明[1] 候勇[1] 

机构地区：[1]安徽医科大学医学生物工程教研室,合肥230032 [2]安徽医科大学 [3]首都医科大学宣武医院,2003级北京100053

出　　处：《淮海医药》2008年第1期1-3,共3页Journal of Huaihai Medicine

摘　　要：目的用体外培养的传代细胞研究三七总皂甙对大鼠肝星状细胞增殖的影响。方法分别将0 mg/ml、0.01 mg/ml、0.05 mg/ml、0.25 mg/ml、1.00 mg/ml和1.25 mg/ml浓度的三七总皂甙作用于HSC-T6细胞,用MTT比色法研究三七总皂甙对细胞增殖的影响。结果培养24 h和48 h后,0.25 mg/ml,1.00 mg/ml和1.25 mg/mI浓度的三七总皂甙对HSC-T6细胞增殖有明显的抑制作用(P<0.05);0 mg/mI、0.01 mg/ml、0.05 mg/ml、0.25 mg/ml,1.00 mg/ml和1.25 mg/ml浓度的三七总皂甙对HSC-T6细胞增殖抑制率呈线性关系,并有明显剂量效应关系,经相关回归分析,培养24 h后,Y=0.486402X+0.037362,r=0.982181,P<0.002;培养48 h后,Y=0.526087X+0.071327,r=0.992046,P<0.001。结论三七总皂甙对HSC-T6细胞增殖有明显抑制作用。Objective To probe the effect of PNS on the proliferation model of HSC-T6 cells. Mechods 5 × 10 per well HSC-T6 cells were seeded in 96 wells and incubated with mixed rear containing 10% NBS and 3% rat serum in the presence or absence of PNS（0. 01 mg/ml,0. 05 mg/ml,0. 25 mg/ml,1.00 mg/ml and 1.25 mg/ml for 24 and 48 hours to evaluate the collagen synthesis,which was assayed by MTT. Results PNS （ 0. 25 - 1.25 mg/ml） inhibited proliferation of HSC-T6 cells stimulated by mixed rear（ P 〈 0. 05 ）. A 24h incubation with PNS at concentration of 0. 01 - 1.25 mg/ml showed Y = 0. 037362 ＋ 0. 486402X, r = 0. 982181 ,P 〈 0. 002 ,while a 48h incubation with PNS at concentration of 0. 01 - 1.25 mg/ml resulted in Y = 0. 071327 ＋ 0. 526087X, r = O. 992046 ,P 〈 0. 001. Conclusion PNS inhibits proliferation of HSC-T6 cells.

关 键 词：肝星状细胞 细胞增殖 三七总皂甙 

分 类 号：R285.5[医药卫生—中药学]