粘质沙雷氏菌抗铜基因的克隆及性质研究  

Cloning and characterization of the copper resistance gene of Serratia marcescens KMR-3 strain

作　　者：周长涛[1] 季秀玲[1] 林连兵[1] 井申荣[1] 谢莹[1] 魏云林[1] 

机构地区：[1]昆明理工大学生物工程技术研究中心,云南昆明650224

出　　处：《云南大学学报（自然科学版）》2008年第1期92-97,共6页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：通过构建粘质沙雷氏菌KMR-3菌株的基因组DNA文库,克隆到了与该菌的铜抗性相关的基因,并对其部分特性进行了研究.结果表明克隆到的铜抗性基因所编码的蛋白属于CutF蛋白,由194个氨基酸编码,与莫氏耶尔森氏菌(Yersinia mollaretii)ATCC 43969抗铜脂蛋白NlpE同源性最高,达到70%,并对该基因的调控序列(启动子、终止子、SD序列及转录起始位点)进行了分析.Copper-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned copper-resistant gene encoded a protein product of 194 amino acids that belonged to CutF protein family and showed 70% similarity to uncharacterized lipoproteinclpE in- volved in copper resistance of Yersinia mollaretii ATCC 43969. Regulation elements including promoter, terminator, SD sequence and transcription start site were also identified in inserted fragment.

关 键 词：粘质沙雷氏菌 抗性基因 克隆 

分 类 号：Q785[生物学—分子生物学]