楸树的初代培养研究  

Study on primary in vitro culture of Catalpa bungei C.A.Mey

作　　者：王改萍[1] 杨燕[1] 祁丽丽[1] 彭方仁[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《江苏林业科技》2007年第6期7-11,共5页Journal of Jiangsu Forestry Science & Technology

基　　金：国家科技部"十一五"科技支撑项目"优良抗逆生态和珍贵用材树种新品种选育"(2006BAD01A16);江苏省高技术研究项目"楸树优良无性系及苗木标准化生产技术研究"(BG2006319)

摘　　要：以楸树的4个品种（类型）（圆基长果楸、豫楸1号、豫楸2号和梓树）为材料进行组织培养研究，试验结果表明：（1）通过豫楸1号和圆基长果楸在MS、1／2MS、N6、WPM、B5 5种基本培养基上的诱导率和启动时间确定了该实验的最佳培养基为N6，其诱导率达80％，接种后2d即启动。（2）外植体的消毒方式采用75％的酒精和0．1％HgCl2双步消毒，4个品种（类型）中豫楸2号的成活率最高，污染率最小。而不同品种（类型）最适消毒时间分别为：圆基长果楸5min、豫楸1号8min、豫楸2号7min、梓树9．5min。（3）大田栽培的圆基长果楸的污染率是温室栽培下的4～6倍，而成活率仅为温室栽培下的1／4，温室栽培的材料更适于进行组织培养。（4）豫楸1号在30d内的污染率动态变化表明，外植体在接种7d后出现污染，而主要污染发生在接种后的第14d和第21d。A study of the tissue culture on 4 varieties of Catalpa （including Yuanjichangguoqiu,Yuqiu-1, Yuqiu-2, and Catalpa ovata） showed that the best medium for tissue culture was N6 （ N6 ＋ 6-BA1.5 mg/L ＋ NAA 0.01 mg/L ＋ sucrose 30 g/L ＋ agar 7g/L） among 5 basic media of MS, 1/2MS,N6 ,WPM and B5 ,based on the inductive rate and the initial time of inductivity of Yuqiu-1 and Yuanjichangguoqiu,which was 80% and two days after incubation respectively. By method of sterilization of 75% ethanol and subsequent 0. 1% HgCl2 to the explants,the contamination rate of Yuqiu-2 was the lowest in the 4 varieties of Catalpa,and the most suitable times of sterilization in 4 varieties were 8min for Yuqiu-1,7min for Yuqiu-2,5 min for Yuanjichangguoqiu,9.5 min for Catalpa ovata respectively. It was also found that the contamination rate of the explants of Yuanjichangguoqiu from the field condition was 4 - 6 times,and the survival rate was only one fourth,compared with that from the greenhouse respectively,indicating that the explants from the greenhouse were better for in vitro culture than those from the field condition. The dynamic contamination of 30 days for Yuqiu-l＇explants illustrated that the explants began to be contaminated in the first week after incubation,and got more serious in the second week and the third week.

关 键 词：楸树 组织培养 培养基 消毒时间 

分 类 号：S687[农业科学—观赏园艺] Q943.1[农业科学—园艺学]