微生物标志物在土壤污染生态学研究中的应用  

Application of microbial markers in the researches of soil pollution ecology

作　　者：王菲[1] 杨官品[1] 李晓军[2] 刘宛[2] 

机构地区：[1]中国海洋大学生命科学与技术学部,山东青岛266003 [2]中国科学院沈阳应用生态研究,沈阳110016

出　　处：《生态学杂志》2008年第1期105-110,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(20337010);国家重点基础研究发展规划项目(2004CB418506);国家环保局“全国土壤现状调查及污染防治专项”资助

摘　　要：微生物标志物已被广泛用于土壤污染生态毒理学诊断和土壤污染的预防与修复效果的评价。本文综述了在土壤污染生态学研究中广泛应用的微生物标志物及相关技术,介绍了常用的土壤酶活性测定方法。同时,着重阐述了近几年发展起来的PCR-DGGE和PLFA技术及其应用进展和优缺点。建议实践中应联合使用多种方法,综合不同技术和方法的优势,确保评价的科学性。Microbial markers have been widely used in soil eco-toxicological diagnosis, soil pollution control, and contaminated soil remediation assessment. This paper summarized the microbial markers frequently employed in the researches of soil pollution ecology and related technologies, and introduced the commonly used methods tor the determination of soil enzyme activities. The recently developed PCR-DGGE and PLFA techniques, their application advances, as well as their shortcomings and advantages were also demonstrated. It was suggested that multi-methods should be combined in use, so as to integrate the advantages of different techniques and methods, and ensure the scientificity of evaluation.

关 键 词：微生物标志物 土壤酶活性 PCR—DGGE PLFA 

分 类 号：X53[环境科学与工程—环境工程]