依诺沙星分子印迹聚合物的分子识别  

Recognition Properties of Enoxacin Imprinted Polymer

作　　者：曹玺珉[1] 廖玲[1] 杜黎明[1] 

机构地区：[1]山西师范大学分析测试中心,临汾041004

出　　处：《应用化学》2008年第1期43-47,共5页Chinese Journal of Applied Chemistry

基　　金：山西省自然科学基金(2007011027)资助课题

摘　　要：采用分子印迹技术合成了以依诺沙星为模板,以甲基丙烯酸和4-乙烯基吡啶同时为功能单体的分子印迹聚合物。运用平衡结合实验研究了聚合物的吸附特性和选择识别能力。Scatchard分析表明,该分子印迹聚合物在识别依诺沙星分子的过程中存在2类不同的结合位点,高亲和力结合位点的离解常数Kd1=0.22 mmol/L,最大表观结合常数Qmax1=52.2μmol/g;低亲和力结合位点的离解常数Kd2=1.23 mmol/L,最大表观结合常数Qmax2=150.5μmol/g。结合底物的实验结果表明,依诺沙星分子印迹聚合物对依诺沙星呈现较高的吸附性能和选择识别特性。将该印迹聚合物作为固相萃取剂,在人血清依诺沙星的分析中对样品进行了有效的净化和富集。With enoxacin as template molecule, methacrylic acid and 4-rinylpyridine as functional monomers, a molecularly imprinted polymer was prepared via a molecular imprinting technique. The molecularly imprinted polymer was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognition. Scatchard analysis showed that two classes of binding sites were formed in the enoxacin imprinted polymer under the studied concentrations. The dissociation constant（Kd1 ） and the apparent maximum binding capacity（Qmax） were K d1 =0. 22 mmol/L, Qmax1 =52.2 μmol/g for high affinity binding sites and Kd2 = 1.23 mmol/L, Qmax2 = 150. 5 μmol/g for low affinity binding sites, respectively. The experiments of binding different suhstrates indicated that the imprinted polymer possessed a high selectivity and a high recognition ability for enoxacin. When it was used as a solid phase extraction sorbent, the imprined polymer effectively completed the clean-up and enrichment of the sample in the analysis of enoxacin from human serum.

关 键 词：分子印迹聚合物 依诺沙星 分子识别 固相萃取 

分 类 号：O631[理学—高分子化学] O658.2[理学—化学]