β环糊精包合血竭对血小板聚集和血栓形成的影响  

Antiplatelet and Anti-thrombotic Effects of β-cyclodextrin Inclusion of Dragon's Blood

作　　者：杨丽川[1] 陈鹏[2] 雷伟亚[2] 胡建林[1] 陈植和[2] 沈志强[2] 

机构地区：[1]昆明医学院药学院 [2]云南省天然药物药理重点实验室,云南昆明650031

出　　处：《昆明医学院学报》2007年第6期33-38,共6页Journal of Kunming Medical College

基　　金：国家自然基金资助项目(30660212)

摘　　要：目的研究血竭β环糊精包合物(包合血竭,血竭净含量占总重量的20%)对血小板聚集和血栓形成的影响.方法应用小鼠尾静脉注射腺苷二磷酸(ADP)和电刺激大鼠颈动脉血栓形成方法评价包合血竭和血竭抗血栓形成作用;采用Born比浊法测定家兔血小板聚集功能.结果包合血竭对电刺激大鼠颈动脉引起的血栓和小鼠尾静脉注射ADP形成肺栓塞具有明显的对抗作用.包合血竭体外显著抑制花生四烯酸(AA)、ADP及血小板活化因子(PAF)诱导的血小板聚集;包合血竭灌胃均明显降低AA、ADP和PAF诱导的家兔血小板聚集率.结论β环糊精包合血竭在多种血栓模型中均具有较好的抗血栓和抗血小板聚集作用,其作用较血竭更强,能提高血竭的生物利用度.Objective To investigate antithrombotic and antiplatelet effects of Dragon＇s Blood, β-cyclodextrin inclusion of Dragon＇s Blood. Methods The methods of injection of adenosin diphosphate （ADP） into mouse tail vein, electrically stimulated arterial thrombosis in rats were used to evaluate the anti-thrombotic effects of Dragon＇s Blood and 13 -cyclodextrin inclusion of Dragon＇s Blood, respectively. Platelet aggregation （PRP or washed platelets） was tested according to Born＇s method. Results β CYDB significantly protected against thrombosis caused by ADP on mice, and electrical stimulation on rats. In vitro β -cyclodextrin inclusion of Dragon＇s Blood inhibited AA, ADP, PAF-induced platelet aggregation. Conclusions The results suggest that compared with Dragon＇s Blood, β-cyclodextrin inclusion of Dragon＇s Blood has more potent anti-thrombotic effects on the thrombotic models, its mechanisms may be closely related to the inhibition of anti-platelet aggregation. β-cyclodextrin inclusion of Dragon＇s Blood can improve the bioavailability of Dragon＇s Blood.

关 键 词：血竭 Β环糊精 包合血竭 抗血栓形成 抗血小板聚集 

分 类 号：R965.2[医药卫生—药理学]