大鼠脊髓全横断后相关部位的BDNF表达  

The Changes on BDNF Expression in Transected Spinal Cord,Associated Motor Cortex and Skeleton Muscle Following Spinal Cord Injury in Rats

作　　者：王昭君[1] 刘佳[1] 习杨彦彬[1] 郝春光[1] 戴萍[1] 王廷华[1] 

机构地区：[1]昆明医学院神经科学研究所,云南昆明650031

出　　处：《昆明医学院学报》2007年第6期58-61,98,共5页Journal of Kunming Medical College

摘　　要：目的探讨大鼠脊髓全横断损伤后相关部位(大脑皮质、横断脊髓上端、下端和比目鱼肌)脑源性神经营养因子(BDNF)mRNA的表达变化.方法首先建立正常对照组和实验组模型,其中实验组又依照大鼠全横断脊髓损伤术后1、3、7、14 d不同观察时间点分为4个亚组,分别抽提横断脊髓上端及其相连的大脑皮质、横断脊髓下端和比目鱼肌组织中总RNA,用RT-PCR技术检测BDNF mRNA的表达,以β-actin为参照确定各时相BDNF mRNA的相对水平.结果(1)大鼠脊髓全横断损伤后,BDNF mRNA在大脑皮质、脊髓横断上、下端和肌肉中均有表达,且在正常对照组中大脑皮质的BDNF mRNA表达少于脊髓横断上、下端(P<0.05);(2)手术后14 d时肌肉组织中BDNF mRNA的表达较手术后1 d和3 d组均有明显增加(P<0.05),说明大鼠脊髓全横断损伤14 d时,脊髓下端相连的骨骼肌组织中的BDNF mRNA表达上调.结论BDNF在骨骼肌表达增加,提示BDNF可能参与维持骨骼肌的功能,或者转运入脊髓下端对下位运动神经元发挥营养作用.Objective To explore the changes on BDNF mRNA in transected spinal cord and associated motor cortex and skeleton muscle following cord injury in rats. Methods 20 adult Sprague Dawleys rats were performed spinal cord transected operation at T11 level, then rats in each group （n = 5） were sacrificed on 1, 3, 7 and 14 days post operation respectively. Other 5 rats were used as normal control without operation. The tissues from the rostral, caudal segments near injury site, cerebral cortex and linea obliqua tibiae were harvested. Total RNA was extracted with Trizol reagent separately. The BDNF mRNA expression in each group was detected by RT-PCR. Results （1） BDNF positive bands were seen in the tissues of the rostral, caudal segments near injury site, cerebral cortex and linea obliqua tibiae. Moreover, BDNF level in cerebral cortex is more than in the spinal cord at normal control （P 〈 0.05） . （2） BDNF expression in 14 days post operation significantly increased in the tissue of linea obliqua tibiae, comp^ed with that of 1 days post operation and 3 days post operation groups （P 〈 0.05） However, there was no significant difference on BDNF expression among other groups. Conclusions BDNF mRNA expression is detected in transected spinal cord injury and associated tissues, which indicate that BDNF might be related to the physiological function in rat spinal cord and associated tissues. However, following spinal cord transected injury, BDNF expression increas only in muscular tissue at 14 days post operation, which indicate that BDNF may be involved in either the control of muscular activity or the function of in spinal motor neurons by transport mechanism.

关 键 词：脊髓损伤 BDNF MRNA RT-PCR 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]