云芝多糖B对血管紧张素Ⅱ诱导的巨噬细胞膜糖胺聚糖和细胞内谷胱甘肽含量变化的影响  

Effect of Coriolus versicolor polysaccharide B on membrane glycosaminoglycans and cellular glutathione changes in RAW264.7 macrophages exposed to angiotensin Ⅱ

作　　者：娄宁[1] 马刚[1] 汪道峰[1] 朱志蔚[1] 苏全冠[1] 方翼[1] 

机构地区：[1]中山大学附属肿瘤防治中心重症监护治疗科/华南肿瘤学国家重点实验室,广东广州510060

出　　处：《南方医科大学学报》2007年第12期1824-1826,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(39700177)~~

摘　　要：目的探讨野生云芝多糖水溶性新组分CVP-B对血管紧张素Ⅱ(AngⅡ)诱导的RAW264.7巨噬细胞糖胺聚糖(GAG)表达及细胞内还原型谷胱甘肽(GSH)的作用。方法用超速离心法分离巨噬细胞膜,用1,9-二甲基亚甲基兰法测定CVP-B对AngⅡ诱导RAW264.7巨噬细胞膜GAG表达的影响;同时应用荧光分光光度法测定CVP-B对AngⅡ诱导RAW264.7巨噬细胞内GSH变化的影响。结果AngⅡ(1μmol/L)刺激RAW264.7细胞,细胞膜GAG含量升高115%;随着CVP-B浓度升高(1,10,50μg/ml),AngⅡ(1μmol/L)诱导的细胞膜GAG分别降低13%、43%(P<0.01)及52%(P<0.01)。同时,AngⅡ(1μmol/L)刺激RAW264.7细胞,细胞内GSH活性降低69%(P<0.01);随着CVP-B浓度升高(1,10,50μg/ml),AngⅡ(1μmol/L)诱导的GSH活性分别升高11%、104%(P<0.01)及168%(P<0.01)。结论CVP-B能明显抑制AngⅡ氧化应激诱导的RAW264.7巨噬细胞膜GAG表达。Objective To investigate the effect of Coriolus versicolor polysaccharide B （CVP-B） on increased membrane glycosaminoglycans （GAG） expression and intracellular glutathione （GSH） of RAW264.7 macrophages exposed to angiotensin Ⅱ （Ang Ⅱ）. Methods The plasma membrane of RAW264.7 macrophages exposed to Ang Ⅱ treatment was isolated by ultracentrifugation, and the membrane GAG expression was analyzed using 1, 9-dimethylmethylene blue （DMMB） spectrophotometric assay for sulfated GAG. The intraceUular reduced GSH was determined using fluorophotometry. Results The GAG content in the macrophage membranes increased by up to 54% following cell exposure to 1.0 μmol/L Ang Ⅱ, whereas in presence of 1.0μmo/l Ang Ⅱ, CVP-B at 1, 10, and 50μg/ml decreased the GAG content by 13%, 43% （P〈0.01）, and 52% （P〈0.01）, respectively. The macrophage GSH activity decreased by 69% following incubation with 1.0μmol/L Ang II for 24 h, and CVP-B treatment at 1, 10, and 50 μg/ml in presence of 1.0 μmo/L Ang Ⅱ resulted in significant increment of GSH activity by 31% （P〈0.05）, 104% （P〈0.01）, and 168% （P〈0.01）, respectively. Conclusion These data provide the first evidence that CVP-B inhibits elevated GAG expression in RAW264.7 macrophage membrane induced by Ang Ⅱ.

关 键 词：云芝 血管紧张素Ⅱ 巨噬细胞 糖胺聚糖 谷胱甘肽 

分 类 号：R285[医药卫生—中药学]