复智散HPLC-UV/ELSD指纹图谱的研究  

Fingerprinting of Fuzhisan by high-performance liquid chromatography with ultraviolet and evaporative light scattering detector

作　　者：赵敬堃[1] 王德生[1] 段淑荣[1] 王建秀[1] 白晶[2] 李文兰[2] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,黑龙江哈尔滨150001 [2]哈尔滨商业大学药物研究所,黑龙江哈尔滨150086

出　　处：《南方医科大学学报》2007年第12期1830-1833,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30670726);黑龙江省教育厅科学技术研究资助项目(11521137)~~

摘　　要：目的建立中药复智散提取物HPLC-UV/ELSD指纹图谱。方法采用HPLC-UV/ELSD串联检测技术,应用AlltechC18(5μm,4.6mm×250mm)色谱柱;流动相为乙腈(A)-0.1%乙酸水溶液(B),线性梯度时间洗脱程序(A)为:0,12%;25,20%;30,20%;75,30%;105,40%;120,80%;130,12%;流速:1.0ml/min;柱温:30℃;检测波长:335nm;飘移管温度80℃;氮气压力25psi。运用指纹图谱相似度评价软件,对12批复智散提取物指纹图谱进行相似度计算。结果建立中药复智散提取物指纹图谱并鉴定其5种主要成分;对UV与ELSD图谱互补性进行分析;12批复智散提取物指纹图谱相似度良好。结论该指纹图谱能同时反映复智散复方中主要化学组分特征,一次进样,可全面、稳定、有效地进行复方中药复智散的质量监督与评价。Objective To establish a method for fingerprinting of Fuzhisan （FZS, a traditional Chinese medicinal preparation） using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector （HPLC-UV/ELSD） to allow simultaneous determination of 5 major constituents in the preparation. Methods HPLC-UV/ELSD analysis was performed on water AlltechC18 column （5 um, 4.6 mm×250mm） with a mixture of acetonitrile （A） and 0.1% acetice acid water （B） as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min and the column temperature at 30℃. The detective wavelength was 335nm, drift tube temperature was 80℃, pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software. Results The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS. Conclusions The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.

关 键 词：高效液相色谱 紫外/蒸发光散射检测 复智散 指纹图谱 

分 类 号：R286[医药卫生—中药学]