大剂量5-FU致肠黏膜严重损伤时肠上皮细胞PCNA的表达  

Expression of proliferating cell nuclear antigen in severely damaged small intestinal mucosa due to high-dose 5-FU exposure

作　　者：罗育其[1] 吴承堂[1] 闻英[1] 廖康雄[1] 张军花[2] 

机构地区：[1]南方医科大学南方医院普通外科,广州510515 [2]南方医科大学南方医院手术室,广州510515

出　　处：《南方医科大学学报》2007年第12期1860-1862,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(032901)~~

摘　　要：目的探讨大剂量5-FU致肠黏膜严重损伤时肠上皮细胞增殖细胞核抗原(PCNA)的表达及其意义。方法成年C57BL/6J小鼠40只,分为对照组(n=8)和实验组(n=32)。对照组给予腹腔注射PBS,实验组给予腹腔注射大剂量5-FU(150mg/kg.d,连续五d),分别于处理后第1、3、5天,剖杀小鼠取小肠,常规HE染色,免疫组化检测PCNA的表达。结果与对照组比较,腹腔注射大剂量5-FU后,小鼠肠黏膜遭受严重破坏,黏膜萎缩,绒毛、隐窝结构消失;免疫组化结果显示PCNA表达增加,PCNA指数显著增高(P<0.01)。结论腹腔注射大剂量5-FU后,肠黏膜细胞中表达PCNA的肠上皮细胞比例增加,可能与肠黏膜损伤后修复有密切的关系。Objective To detect the expression of proliferating cell nuclear antigen （PCNA） in severely damaged intestinal mucosa due to high-dose 5-FU exposure. Methods Thirty-two adult C57BL/6J mice were subjected to daily intraperitoneal high-dose 5-FU injection at 150 mg/kg for 5 consecutive days, and on days 1, 3, and 5, the mice were sacrificed to obtain the small intestinal tissue for HE straining and immunohistochemistry for detecting PCNA expression. Another 8 mice with intraperitoneal PBS injection served as the control group. Results High-dose 5-FU exposure of the mice resulted in severe intestinal mucous damage, with complete destruction of the villi and crypts and significantly increased cells positive for PCNA expression （P〈0.01）. Conclusions High-dose 5-FU treatment can significantly increase the PCNA index, and the cells expressing PCNA can be closely associated with regeneration of the severely damaged mucosa due to the exposure.

关 键 词：肠黏膜 肠上皮干细胞 PCNA 5-FU 

分 类 号：R96[医药卫生—药理学]