杉木第2代育种群体优良基因型的RAPD遗传变异(英文)  

Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers

作　　者：李梅[1] 施季森[2] 李发根[1] 甘四明[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广州510520 [2]南京林业大学国家林业局林木遗传与基因工程重点实验室,南京210037

出　　处：《林业科学》2007年第12期50-55,共6页Scientia Silvae Sinicae

基　　金：Funded by a national key project of China (2002BA515B0101)

摘　　要：利用RAPD标记研究选自杉木第2代育种试验林、拟纳入高世代育种的182个优良基因型的遗传变异。29条随机引物扩增出311条谱带,其中多态性谱带245条(78.8%)。聚类分析表明,182个优良基因型可聚为11类。AMOVA分析表明,96.8%的遗传变异存在于第2代育种试验林内,而试验林间的遗传变异虽然显著(Φst=0.032,P<0.001),但比例较低(3.2%)。研究结果表明,杉木第2代育种群体存在高水平的遗传多样性,显示了进一步育种的良好潜力。RAPD markers were used to characterize the genetic diversity and relationships among 182 elite genotypes selected from second-generation trials as breeds for advanced generation breeding in Chinese Fir （ Cunninghamia lanceolata ）. Twentynine 10-mer primers generated a total of 311 fragments,245 （78.8%） of which were polymorphic. Cluster analyses allowed for all 182 genotypes to be grouped into 11 clusters. AMOVA analyses showed that a high proportion （96.8%） of total genetic di-versity resided within the field trials from which the elite genotypes were selected, whereas genetic diversity among field trials was low but significant （ Фst, = 0.032, P 〈 0.001）. These results demonstrate that high levels of genetic diversity exist within the second-generation breeding population of Chinese Fir and hence suggest that there is good potential for genetic gains from further breeding.

关 键 词：杉木 优良基因型 遗传变异 RAPD 

分 类 号：S718.46[农业科学—林学] S722.33