VEGF纳米粒的制备及药剂学性质研究  

Preparation of VEGF nanoparticles and the researches on its pharmaceutical characteristics

作　　者：王玮[1] 陈玉成[2] 张丹[1] 刘慧娜[1] 宁二娟[1] 张海峰[1] 

机构地区：[1]河南大学药学院,河南开封475004 [2]四川大学华西医院,四川成都610041

出　　处：《河南大学学报（医学版）》2007年第4期11-13,共3页Journal of Henan University:Medical Science

摘　　要：目的：将VEGF制备为纳米粒，以提高VEGF在体内的稳定性，解决VEGF半衰期短等问题，并对所制备的VEGF纳米粒进行药剂学性质研究。方法：以无毒、可生物降解的聚氰基丙烯酸酯为材料，采用乳化聚合法制备VEGF纳米粒，对其外观形态、粒径分布、包封率和载药量以及体外释放进行考察。结果：制备了纳米粒胶体溶液及其冻干品：电子透射显微镜下观察纳米粒形态，外形为粒径比较均匀的球状或近球状的纳米粒，平均粒径为90nm，粒径范围为50～150nm；包封率大于85％，载药量约为42％，冻干品的再分散性良好。结论：本研究制备的VEGF纳米粒，包封率和载药量相对较高，质量稳定，重现性好。Objective： To study the preparation method of VEGF nanoparticles and its pharmaceutical characteristics in order to improve its stability in vivo and increase its therapeutical effect, Methods： The nanoparticles were prepared by emulsion polymerization method. The morphology of VEGF nanoparticles, the drug loading, the entrapment efficiency and the particle size distribution were studied, Results： The VEGF nanoparticles were acolloid solution. More than 85% of initial VEGF contents were entrapped into the VEGF nanoparticles. The result of electron micrography and the size distribution showed that the VEGF nanoparticles were small spherical or similar to spherical vesicles. The mean diameter of VEGF nanoparticles was 90 nm with the range of 50 - 150 nm , and the drug loading was 42%. The redispersibility of freeze drying powder was very good, Conclusion： The VEGF is highly entrapped into nanoparticles with good reproducibility and stability.

关 键 词：VEGF 聚氰基丙烯酸酯 纳米粒 血管内皮细胞 

分 类 号：R927.2[医药卫生—药学]