HPLC法测定凉血化斑颗粒剂中绿原酸的含量  

Determination of Chlorogenic Acid by HPLC in Liangxuehuaban Granules

作　　者：任荣[1] 李正翔[1] 李彬[1] 孟冲[1] 董伟林[2] 

机构地区：[1]天津医科大学总医院,天津市300052 [2]天津医科大学实验中心,天津市300070

出　　处：《中国医院用药评价与分析》2007年第6期441-443,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的：采用HPLC法测定凉血化斑颗粒中绿原酸的含量。方法：用十八烷基键合硅胶柱，乙腈-0．4％磷酸（13：87）为流动相：检测波长为327nm。结果：绿原酸在5～200μg·mL^-1范围内线形关系良好（r=0.9999），平均回收率为92．13±1．28％，日间误差RSD小于2％。结论：本方法具有快速、简便、灵敏、准确、经济等特点，适用于凉血化斑颗粒剂的质量控制。OBJECTIVE： To determine chlorogenic acid of Liangxuehuaban gwanules by high performance liquid chromatography. METHODS ： Using C 18column, the mobile phase consisted of CH3 CN - 0.4 % H3 PO4 ; The analysis was taken at 327nm. RESULTS：The linear range was 5 - 200μg·mL^-1. The average recovery was 92.13% （RSD was 1.28%, n = 5）,The relative standard deviations of between- day was less than 2%. CONCLUSION： The method is rapid, simple, sensitiveand accurate. It was suitable for the quality control of Liangxuehuaban.

关 键 词：凉血化斑 红斑狼疮 颗粒剂 高效液相色谱法 

分 类 号：R277.523.4[医药卫生—中医学]